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The pattern of X-chromosome inactivation in the embryonic and extra-embryonic tissues of post-implantation digynic triploid LT/Sv strain mouse embryos

Published online by Cambridge University Press:  14 April 2009

S. Speirs ,
J. M. Cross  and
M. H. Kaufman
S. Speirs
Affiliation:
Department of Anatomy, University Medical School, Teviot Place, Edinburgh, EH8 9AG, UK
J. M. Cross
Affiliation:
Department of Anatomy, University Medical School, Teviot Place, Edinburgh, EH8 9AG, UK
M. H. Kaufman*
Affiliation:
Department of Anatomy, University Medical School, Teviot Place, Edinburgh, EH8 9AG, UK
*
* Corresponding author.
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Spontaneously cycling LT/Sv strain female mice were mated to hemizygous Rb(X.2)2Ad males in order to facilitate the distinction of the paternal X chromosome, and the pregnant females were autopsied at about midday on the tenth day of gestation. Out of a total of 222 analysable embryos recovered, 165 (74·3%) were diploid and 57 (25·7%) were triploid. Of the triploids, 26 had an XXY and 31 an XXX sex chromosome constitution. Both embryonic and extra-embryonic tissue samples from the triploids were analysed cytogenetically by G-banding and by the Kanda technique to investigate their X-inactivation pattern. The yolk sac samples were separated enzymatically into their endodermally-derived and mesodermally-derived components, and these were similarly analysed, as were similar samples from a selection of control XmXp diploid embryos. In the case of the XmXmY digynic triploid embryos, a single darkly-staining Xm chromosome was observed in 485 (82·9%) out of 585, 304 (73·3%) out of 415, and 165 (44·7%) out of 369 metaphases from the embryonic, yolk sac mesodermally-derived and yolk sac endodermally-derived tissues, respectively. The absence of a darkly staining X-chromosome in the other metaphase spreads could either indicate that both X-chromosomes present were active, or that the Kanda technique had failed to differentially stain the inactive X-chromosome(s) present. In the case of the XmXmXp digynic triploid embryos, virtually all of the tissues analysed comprised two distinct cell lineages, namely those with two darkly-staining X-chromosomes, and those with a single darkly staining X-chromosome. Four X-inactivation patterns were consequently observed in this group, namely, (XmXp)Xm, (XmXm)Xp, (Xm)XmXp and XmXm(Xp) in which the inactive X is enclosed in parentheses. The incidence of these various classes varied among the tissues analysed. There was, however, a clear pattern of non-random selective paternal X-inactivation in yolk sac endodermally-derived samples which possessed two inactive X-chromosomes. This finding contrasts with the situation observed in the yolk sac mesodermally-derived and embryonic samples which possessed two inactive X-chromosomes, where the ratio of (XmXm)Xp:Xm(XmXp) was 1:1·20 and 1:1·03, respectively, being clear evidence that random X-inactivation had occurred in these tissues.

Type
Research Article
Information
Genetics Research , Volume 56 , Issue 2-3 , October 1990 , pp. 107 - 114
Copyright
Copyright © Cambridge University Press 1990

References

Adler, I. D., Johannisson, R. & Winking, H. (1989). The influence of the Robertsonian translocation Rb(X. 2)2Ad on anaphase I non-disjunction in male laboratory mice. Genetical Research 53, 77–86.CrossRefGoogle ScholarPubMed
Beatty, R. A. (1957). Parthenogenesis and Polyploidy in Mammalian Development. London: Cambridge University Press.Google Scholar
Beatty, R. A. (1978). The origin of human triploidy: an integration of qualitative and quantitative evidence. Annals of Human Genetics 41, 299–314.CrossRefGoogle ScholarPubMed
Boué, J., Boué, A. & Lazar, P. (1975). The epidemiology of human spontaneous abortions with chromosomal anomalies. In Aging Gametes: Their Biology and Pathology, (ed. Blandau, R. J.), pp. 330–348. Basel: S. Karger.Google Scholar
Carr, D. H. (1971 a). Genetic basis of abortion. Annual Review of Genetics 5, 65–80.Google Scholar
Carr, D. H. (1971 b). Chromosome studies in selected spontaneous abortions. Polyploidy in man. Journal of Medical Genetics 8, 164–174.Google Scholar
Dyban, A. P. & Baranov, V. S. (1987). Cytogenetics of Mammalian Embryonic Development. Oxford: Clarendon Press.Google Scholar
Endo, S., Takagi, N. & Sasaki, M. (1982). The late replicating X chromosome in digynous mouse triploid embryos. Developmental Genetics 3, 165–176.Google Scholar
Evans, E. P., Burtenshaw, M. D. & Ford, C. E. (1972). Chromosomes of mouse embryos and newborn young, preparations from membranes and tail tips. Stain Technology 47, 229–234.Google Scholar
Frels, W. I. & Chapman, V. M. (1980). Expression of the maternally derived chromosome in the mural trophoblast of the mouse. Journal of Embryology and Experimental Morphology 56, 179–190.Google Scholar
Kanda, N. (1973). A new differential technique for staining the heteropycnotic X-chromosome in female mice. Experimental Cell Research 80, 463–467.Google Scholar
Kaufman, M. H. & Hewlett, S. K. (1986). The ovulation and activation of primary and secondary oocytes in LT/Sv strain mice. Gamete Research 14, 255–264.CrossRefGoogle Scholar
Kaufman, M. H. & Speirs, S. (1987). The postimplantation development of spontaneous digynic triploid embryos in LT/Sv strain mice. Development 101, 383–391.Google Scholar
Kaufman, M. H., Lee, K. K. H. & Speirs, S. (1989). Influence of diandric and digynic triploid genotypes on early mouse embryogenesis. Development 105, 137–145.Google Scholar
Lin, C. C., De Braekeleer, M. & Jamro, H. (1985). Cytogenetic studies in spontaneous abortion: the Calgary experience. Canadian Journal of Genetics and Cytology 27, 565–570.CrossRefGoogle ScholarPubMed
Levak-Svajger, B., Svajger, A. & Skreb, N. (1969). Separation of germ layers in presomite rat embryos. Experientia 25, 1311–1313.CrossRefGoogle Scholar
Lyon, M. F. (1961). Gene action in the X-chromosome of the mouse (Mus Musculus L.). Nature 190, 372–373.CrossRefGoogle ScholarPubMed
Lyon, M. F. (1962). X-chromosome inactivation and developmental patterns in mammals. Biological Reviews 47, 1–35.Google Scholar
Lyon, M. F. & Rastan, S. (1984). Parental source of chromosome imprinting and its relevance for X chromosome inactivation. Differentiation 26, 63–67.Google Scholar
Monk, M. (1981). A stem-line model for cellular and chromosomal differentiation in early mouse development. Differentiation 19, 71–76.Google Scholar
Monk, M. & Harper, M. I. (1979). Sequential X chromosome inactivation coupled with cellular differentiation in early mouse embryos. Nature 281, 311–313.CrossRefGoogle ScholarPubMed
Niebuhr, E. (1974). Triploidy in man. Cytogenetical and clinical aspects. Humangenetik 21, 103–125.Google Scholar
O'Neill, G. T. & Kaufman, M. H. (1987). Ovulation and fertilization of primary and secondary oocytes in LT/Sv strain mice. Gamete Research 18, 27–36.Google Scholar
Rastan, S., Kaufman, M. H., Handyside, A. H. & Lyon, M. F. (1980). X-chromosome inactivation in extraembryonic membranes of diploid parthenogenetic mouse embryos demonstrated by differential staining. Nature 288, 172–173.CrossRefGoogle ScholarPubMed
Speirs, S. & Kaufman, M. H. (1989). Analysis of the sex chromosome constitution of digynic triploid mouse embryos. Cytogenetics and Cell Genetics 52, 151–153.Google Scholar
Speirs, S. & Kaufman, M. H. (1990). The effect of maternal age on the incidence of digynic triploidy in LT/Sv strain mice: implications for the ovulation of primary and secondary oocytes in this strain. Journal of Experimental Zoology 253, 83–87.Google Scholar
Takagi, N. & Sasaki, M. (1975). Preferential inactivation of the paternally derived X chromosome in the extraembryonic membranes of the mouse. Nature 256, 640–642.CrossRefGoogle ScholarPubMed
Wake, N., Takagi, N. & Sasaki, M. (1976). Non-random inactivation of X chromosome in the rat yolk sac. Nature 262, 580–581.Google Scholar
West, J. D., Fels, W. I., Chapman, V. M. & Papaioannou, V. E. (1977). Preferential expression of the maternally derived X chromosome in the mouse yolk sac. Cell 12, 873–882.Google Scholar
Whittingham, D. G. & Wales, R. G. (1969). Storage of two-cell mouse embryos in vitro. Australian Journal of Biological Sciences 22, 1065–1068.CrossRefGoogle ScholarPubMed