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Origin of rare Ha-ras alleles: relationship of VTR length to a 5′ polymorphic Xho I site

Published online by Cambridge University Press:  14 April 2009

Glenn D. Baxter ,
Nicholas K. Hayward ,
Russell J. Collins  and
Martin F. Lavin
Glenn D. Baxter
Affiliation:
Queensland Institute of Medical Research, Herston, Brisbane 4006., Australia
Nicholas K. Hayward
Affiliation:
Queensland Institute of Medical Research, Herston, Brisbane 4006., Australia
Russell J. Collins
Affiliation:
Division of Immunology, Department of Pathology, Royal Brisbane Hospital, Brisbane, 4006
Martin F. Lavin*
Affiliation:
Queensland Institute of Medical Research, Herston, Brisbane 4006., Australia
*
*Corresponding author.
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Amongst the four common Ha-ras alleles in both controls and cancer patients, we detected the presence of a polymorphic Xho I site associated specifically with the 6·6 and 7·7 kb Bam HI fragments but absent from the 7·1 and 8·2 kb alleles, as recently reported by others. We have extended this study and report here, the consistent appearance of this Xho I site in unusual alleles close in size to the two common alleles of 6·6 and 7·7 kb, in control lymphoblastoid DNA samples in a variety of tumor DNAs. Unusual alleles grouped around the 7·1 and 8·2 kb common alleles on the other hand, did not possess the Xho I site. The consistent presence of the Xho I site polymorphism, in the unusual Ha-ras alleles surrounding the 6·6 and 7·7 kb common alleles and its absence in alleles around the 7·1 and 8·2 kb common alleles, suggests that the unusual ones are derived from the corresponding common alleles to which they are closest in size.

Type
Research Article
Information
Genetics Research , Volume 54 , Issue 2 , October 1989 , pp. 149 - 153
Copyright
Copyright © Cambridge University Press 1989

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