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The Ontogeny of γ-crystallin mRNAs in CatFraser Mice

Published online by Cambridge University Press:  14 April 2009

Maciej Kuliszewski ,
Jim Rupert  and
R. Gold
Maciej Kuliszewski
Affiliation:
Department of Medical Genetics, University of Toronto, Toronto, Ontario, Canada M5S 1A8
Jim Rupert
Affiliation:
Department of Medical Genetics, University of Toronto, Toronto, Ontario, Canada M5S 1A8
R. Gold*
Affiliation:
Department of Medical Genetics, University of Toronto, Toronto, Ontario, Canada M5S 1A8
*
* Corresponding author.
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Summary

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Mice which are either homozygous or heterozygous for the CatFraser mutation have ocular cataracts accompanied by selective reduction of the γ-crystallins, a homologous family of proteins present in the lens and encoded by a family of tightly linked genes. We measured the concentrations of four different mRNAs, each encoding a different γ-crystallin, in the lenses of homozygous CatFraser mice and in normal controls at various stages of development by preparing Northern blots from lens RNA, probing with RNAs complementary to each of the four messages and densitometry of the bands thus generated. The results show that, for each of these messages, the ontogenetic patterns observed in normal mice are retained in the mutant, but at much lower concentrations.

Type
Research Article
Information
Genetics Research , Volume 52 , Issue 1 , August 1988 , pp. 45 - 49
Copyright
Copyright © Cambridge University Press 1988

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