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Nuclear and cytoplasmic cross-resistance and correlated sensitivity to DNA intercalating drugs in a petite-negative yeast

Published online by Cambridge University Press:  14 April 2009

Esteban Celis ,
Jaime Mas  and
Aurora Brunner
Esteban Celis
Affiliation:
Departamento de Bioquímica, Facultad de Medicina and Departamento de Biología Experimental, Instituto de BiologíaUniversidad Nacional Autónoma de México, México 20, D.F., México
Jaime Mas
Affiliation:
Departamento de Bioquímica, Facultad de Medicina and Departamento de Biología Experimental, Instituto de BiologíaUniversidad Nacional Autónoma de México, México 20, D.F., México
Aurora Brunner
Affiliation:
Departamento de Bioquímica, Facultad de Medicina and Departamento de Biología Experimental, Instituto de BiologíaUniversidad Nacional Autónoma de México, México 20, D.F., México
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Ethidium bromide and acriflavin-resistant mutants of petite-negative yeast Kluyveromyces lactis were prepared. One kind of nuclear mutation (EBR1) gave resistance to ethidium bromide and correlated sensitivity towards acriflavin. Another nuclear mutation (EBR2) did not affect ‘natural’ resistance of this yeast towards 15 μM acriflavin. Both nuclear mutations mapped at different loci, suggesting lack of linkage. Cytoplasmic mutants resistant to these two drugs were unstable when grown in complete media with dextrose, reverting to a wild-type resistance genotype. When grown in glycerol-containing media these mutants maintained their cytoplasmic drug-resistance conferring factors.

Type
Research Article
Information
Genetics Research , Volume 25 , Issue 1 , February 1975 , pp. 59 - 69
Copyright
Copyright © Cambridge University Press 1975

References

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