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Non-reciprocal intragenic mitotic recombination in Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

Patricia Tamarah Kelly
Patricia Tamarah Kelly
Affiliation:
Department of Genetics, University of California, Berkeley, California 94720
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X-ray-induced mitotic recombination within the lozenge locus of Drosophila melanogaster was found to be non-reciprocal. Recombination was detected by the presence of faceted spots in a smooth (lozenge) eye. Markers were present so that if recombination were reciprocal, equal numbers of vermilion and apricot-vermilion-coloured faceted spots would be formed. Of 19 faceted spots found among more than 60000 eyes examined, 10 were vermilion, 7 variegated (some vermilion and some apricot-vermilion-coloured facets), and 2 probably variegated. None was uniformly apricot-vermilion in colour. Four mechanisms for the formation of faceted spots are discussed: double reciprocal recombination, misrepair or gene conversion before chromatid replication, misrepair or gene conversion after chromatid replication, and misrepair or gene conversion followed by chromosome loss. The evidence most strongly supports the hypothesis that all faceted spots were formed by either misrepair following X-irradiation or gene conversion and were originally vermilion in colour. Subsequent chromosome loss from these vermilion cells led to the production of variegated spots. Variegated spots were generally smaller than those uniformly vermilion in colour, indicating that chromosome loss may occur with greater frequency in older irradiated larvae.

Type
Research Article
Information
Genetics Research , Volume 23 , Issue 1 , February 1974 , pp. 1 - 12
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Ballantyne, G. H. & Chovnick, A. (1971). Gene conversion in higher organisms: Non-reciprocal recombination events at the rosy cistron in Drosophila melanogaster. Genetical Research 17, 139–149.CrossRefGoogle ScholarPubMed
Chovnick, A. (1961). The garnet locus in Drosophila melangaster. I. Pseudoallelism. Genetics 46, 493–507.CrossRefGoogle Scholar
Chovnick, A., Ballantyne, G. H., Ballie, D. L. & Holm, D. G. (1970). Gene conversion in higher organisms: Half-tetrad analysis of recombination within the rosy cistron of Drosophila melanogaster. Genetics 66, 315–329.CrossRefGoogle ScholarPubMed
Clayton, F. E. (1954). The development of the compound eyes of lozenge alleles in D. melanogaster. The University of Texas Publications, no. 5422, pp. 210–243.Google Scholar
Ephrussi, B. & Herold, J. L. (1944). Studies of eye pigments of Drosophila. I. Methods of extraction and quantitative estimation of the pigment components. Genetics 29, 148–175.CrossRefGoogle ScholarPubMed
Finnerty, V. G., Duck, P. & Chovnick, A. (1970). Studies on genetic organization in higher organisms. II. Complementation and fine structure of the maroon-like locus of D. melanogaster. Proceedings of the National Academy of Sciences, U.S.A. 65, 939–946.CrossRefGoogle Scholar
Fogel, S. & Mortimer, R. K. (1971). Recombination in yeast. Annual Review of Genetics 5, 219–236.CrossRefGoogle ScholarPubMed
Green, M. M. (1948). Red eye pigment determination in the lozenge allelie series of D. melanogaster. American Naturalist 82, 188–192.CrossRefGoogle Scholar
Green, M. M. & Green, K. C. (1956). A cytogenetic analysis of the lozenge pseudoalleles in Drosophila. Zeitschrift fur induktive Abstammungs-und Vererbungslehre 87, 708–721.Google ScholarPubMed
Hartman, H. & Hayes, T. L. (1971). Scanning electron microscopy of Drosophila. Journal of Heredity 62, 41–44.CrossRefGoogle ScholarPubMed
Hexter, W. M. (1963). Nonreciprocal events at the garnet locus in D. melanogaster. Proceedings of the National Academy of Sciences, U.S.A. 50, 372–379.CrossRefGoogle Scholar
Hexter, W. M. (1964). Gene conversion in D. melanogaster. Atti Associazione Genetica Italiana 9, 195–206.Google Scholar
Lindsley, D. L. & Grell, E. H. (1967). Genetic variations of Drosophila melanogaster. Carnegie Institution of Washington Publication no. 627.Google Scholar
Painter, R. B. (1971). Repair of DNA in mammalian cells. Current Topics in Radiation Research 7, 45–70.Google Scholar
Stern, C. (1936). Somatic crossing over and segregation in Drosophila melanogaster. Genetics 21, 625–730.CrossRefGoogle ScholarPubMed
Stern, C. (1969). Somatic recombination within the white locus of Drosophila melanogaster. Genetics 62, 573–581.CrossRefGoogle ScholarPubMed
Valencia, J. I. & Plaut, W. (1969). X-ray-induced DNA synthesis in polytene chromosomes. Journal of Cell Biology 43, 151A (abstract).Google Scholar