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Nitrite toxicity in Aspergillus nidulans: a new locus in a proA1 pabaA6 yA2 strain

Published online by Cambridge University Press:  14 April 2009

Sandra R. C. Pombeiro ,
Nilce M. Martinez-Rossi  and
Antonio Rossi
Sandra R. C. Pombeiro
Affiliation:
Departamento de Genética, Faculdade de Medicina de Ribeirão Preto-USP, 14.100 Ribeirão Preto, SP, Brazil
Nilce M. Martinez-Rossi*
Affiliation:
Departamento de Genética, Faculdade de Medicina de Ribeirão Preto-USP, 14.100 Ribeirão Preto, SP, Brazil
Antonio Rossi
Affiliation:
Departamento de Genética, Faculdade de Medicina de Ribeirão Preto-USP, 14.100 Ribeirão Preto, SP, Brazil
*
* To whom correspondence should be addressed.

Summary

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This report describes the existence of a mutation in a proA1 pabaA6 yA2 strain of Aspergillus nidulans, which prevents it growing on elevated concentrations of nitrite. The nitrite toxicity marker (nihA) resulted from a mutation in a single gene representing a new locus in linkage group I, and behaved as a recessive character. The observed recombination frequencies between nihA and proA1 and between nihA and pabaA6 were about 1 and 11%, respectively. Nitrite toxicity was not due to a defect in nitrite reductase, because the mutant grew well on nitrate. Furthermore, ammonium protected both nis-5 and niiA− strains against nitrite toxicity, which was not observed for the nihA strain. Since growth of the mutant was inhibited more by a mixture of ammonium and nitrite, compared to nitrite alone, it is proposed that the proA1 pabaA6 yA2 strain has a mutation leading to increased efficiency in nitrite uptake, which is not repressed by ammonium.

Type
Short Papers
Information
Genetics Research , Volume 41 , Issue 2 , April 1983 , pp. 203 - 207
Copyright
Copyright © Cambridge University Press 1983

References

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