Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-05T16:47:14.519Z Has data issue: false hasContentIssue false
  • English
  • Français

A new sex factor of Pseudomonas aeruginosa

Published online by Cambridge University Press:  14 April 2009

J. M. Pemberton  and
B. W. Holloway
J. M. Pemberton
Affiliation:
Department of Genetics, Monash University, Clayton, Victoria 3168, Australia
B. W. Holloway
Affiliation:
Department of Genetics, Monash University, Clayton, Victoria 3168, Australia
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Of 150 wild-type strains of Pseudomonas aeruginosa examined, 48 formed recombinants when mated to P. aeruginosa strain PAO FP− and hence presumably possess sex factors. Three different types of sex factor were distinguished by the pattern of transfer of particular markers in different regions of the chromosome and by the ability to confer resistance to mercury in strain PAO. One new sex factor, FP39, was studied in detail, and while similar to the previously studied FP2 in terms of transfer kinetics, natural stability and resistance to curing by acridines, it differed from FP2 in promoting chromosome transfer from a site 10 min to the left of the FP2 origin and in showing apparently aberrant entry kinetics for a leucine marker situated 48 min from the FP2 origin. This was due to FP39 having a genetic determinant either for a structural gene of leucine biosynthesis or a specific suppressor gene for this locus. PAO strains carrying both FP2 and FP39 were unstable for both sex factors, suggesting a relationship between them.

Type
Research Article
Information
Genetics Research , Volume 21 , Issue 3 , June 1973 , pp. 263 - 272
Copyright
Copyright © Cambridge University Press 1973

References

REFERENCES

Holloway, B. W. & Jennings, P. A. (1958). An infectious fertility factor in Pseudomonas aeruginosa. Nature, London 181, 855–856.CrossRefGoogle ScholarPubMed
Holloway, B. W., Egan, J. B. & Monk, M. (1960). Lysogeny in Pseudomonas aeruginosa. Australian Journal of Experimental Biology and Medical Science 38, 321–330.CrossRefGoogle ScholarPubMed
Holloway, B. W., Krishnapillai, V. & Stanisich, V. A. (1971). Pseudomonas genetics. Annual Review of Genetics 5, 425–446.CrossRefGoogle ScholarPubMed
Kemper, J. & Margolin, P. (1969). Suppression by gene substitution for the leuD gene of Salmonella typhimurium. Genetics 63, 263–279.CrossRefGoogle ScholarPubMed
Loutit, J. S. (1969 a). Investigation of the mating system of Pseudomonas aeruginosa strain 1. IV. Late markers. Genetical Research, Cambridge 13, 91–98.CrossRefGoogle Scholar
Loutit, J. S. (1969 b). Investigation of the mating system of Pseudomonas aeruginosa strain 1. V. The effect of N-methyl-N′-nitro-N-nitrosoguanidine on a donor strain. Genetical Research, Cambridge 14, 103–109.CrossRefGoogle ScholarPubMed
Loutit, J. S. (1971). Investigation of the mating system of Pseudomonas aeruginosa strain 1. VI. Mercury resistance associated with the sex factor (FP). Genetical Research, Cambridge 16, 179–184.CrossRefGoogle Scholar
Loutit, J. S. & Marinus, M. G. (1969). Investigations of the mating system of Pseudomonas aeruginosa strain 1. II. Mapping of a number of early markers. Genetical Research, Cambridge 12, 37–44.CrossRefGoogle Scholar
Loutit, J. S., Marinus, M. G. & Pearce, L. E. (1968). Investigation of the mating system of Pseudomonas aeruginosa strain 1. III. Kinetic studies on the transfer of sex factor FP. Genetical Research, Cambridge 12, 139–145.CrossRefGoogle Scholar
Novick, R. P. (1969). Extrachromosomal inheritance in bacteria. Bacteriological Reviews 33, 210–263.CrossRefGoogle ScholarPubMed
Pemberton, J. M. & Holloway, B. W. (1972). Chromosome mapping in Pseudomonas aeruginosa. Genetical Research, Cambridge 19, 251–260.CrossRefGoogle ScholarPubMed
Rolfe, B. & Holloway, B. W. (1966). Alterations in host specificity of bacterial deoxyribo-nucleic acid after an increase in growth temperature of Pseudomonas aeruginosa. Journal of Bacteriology 92, 43–48.CrossRefGoogle Scholar
Rolfe, B. & Holloway, B. W. (1969). Host specificity of DNA and conjugation in Pseudomonas aeruginosa. Genetics 61, 341–347.CrossRefGoogle ScholarPubMed
Stanisich, V. A. (1973). Isolation and properties of mutants of the FP2 sex factor of Pseudomonas aeruginosa Genetical Research. (in Press).CrossRefGoogle ScholarPubMed
Stanisich, V. A. & Holloway, B. W. (1969 a). Conjugation in Pseudomonas aeruginosa. Genetics 61, 327–339.CrossRefGoogle ScholarPubMed
Stanisich, V. A. & Holloway, B. W. (1969 b). Genetic effects of acridines on Pseudomonas aeruginosa. Genetical Research, Cambridge 13, 57–70.CrossRefGoogle ScholarPubMed
Stanisich, V. A. & Holloway, B. W. (1971). Chromosome transfer in Pseudomonas aeruginosa mediated by R factors. Genetical Research, Cambridge 17, 169–172.CrossRefGoogle ScholarPubMed
Stanisich, V. A. & Holloway, B. W. (1972). A mutant sex factor of Pseudomonas aeruginosa. Genetical Research, Cambridge 19, 91–108.CrossRefGoogle ScholarPubMed
Vogel, H. J. & Bonner, D. M. (1956). Acetyl-ornithinase of E. coli: partial purification and some properties. Journal of Biological Chemistry 218, 97–116.CrossRefGoogle ScholarPubMed