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New data on the linkage map of Streptomyces coelicolor

Published online by Cambridge University Press:  14 April 2009

David A. Hopwood
David A. Hopwood
Affiliation:
Department of Genetics, The University, Glasgow
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Linkage data, obtained by a combination of selective analysis of haploid recombinants and analysis of segregating heterozygotes, are given for twenty-eight loci in Streptomyces coelicolor A3(2). This brings the total known loci for the organism to thirty-nine. The two linkage groups previously recognized remain separate, and their lengths have been increased to about 60 and 70 recombination units respectively. Whether the two linkage groups correspond to two chromosomes remains an open question.

Three further examples of close linkage of pairs of functionally related loci have been found (a trio of such loci was already known), and three other pairs provide possible examples of the same phenomenon. Some loci which are clustered in Salmonella are unlinked in Streptomyces.

Type
Research Article
Information
Genetics Research , Volume 6 , Issue 2 , July 1965 , pp. 248 - 262
Copyright
Copyright © Cambridge University Press 1965

References

REFERENCES

Anagnostopoulos, C., Borat, M. & Schneider, A. M. (1964). Étude, par transformation, de deux groupes de gènes régissant la biosynthese de l'isoleucine, de la valine et de la leucine chez Bacillus subtilis. C. r. hebd. Séanc. Acad. Sci., Paris, 258, 749–752.Google Scholar
Breed, R. S., Murray, E. G. D. & Smith, N. R. (eds.) (1957). Bergey's Manual of Determinative Bacteriology, 7th ed., pp. 33–34. London: Baillière, Tindall & Cox.Google Scholar
Cairns, J. (1963). The bacterial chromosome and its manner of replication as seen by autoradiography. J. molec. Biol. 6, 208–213.Google Scholar
Demerec, M. (1956). Terminology and nomenclature. Genetic Studies with Bacteria. Publs Carnegie Instn, 612, 1–4.Google Scholar
Falcow, S., Rownd, R. & Baron, L. S. (1962). Genetic homology between Escherichia coli K-12 and Salmonella. J. Bact. 84, 1303–1312.CrossRefGoogle Scholar
Giles, N. H. (1963). Genetic fine structure in relation to function in Neurospora. Proc. 11th Int. Cong. Genet 2, 17–30.Google Scholar
Hartman, P. E., Loper, J. C. & Serman, D. (1960). Fine structure mapping by complete transduction between histidine-requiring Salmonella mutants. J. gen. Microbiol. 22, 323–353.CrossRefGoogle ScholarPubMed
Hayes, W. (1964). The Genetics of Bacteria and their Viruses. Oxford: Blackwell Scientific Publications.Google Scholar
Holloway, B. W., Hodgins, L. & Fargie, B. (1963). Unlinked loci affecting related bio-synthetic steps in Pseudomonas aeruginosa. Nature, Lond. 199, 926–927.CrossRefGoogle Scholar
Hopwood, D. A. (1957). Genetic recombination in Streptomyces coelicolor. J. gen. Microbiol. 16, ii–iii.Google Scholar
Hopwood, D. A. (1959). Linkage and the mechanism of recombination in Streptomyces coelicolor. Ann. N.Y. Acad. Sci. 81, 887–898.Google Scholar
Hopwood, D. A. (1964). Urease production as a genetic marker in Streptomyces coelicolor. Microb. Genet. Bull. 20, 7–8.Google Scholar
Hopwood, D. A. & Sermonti, G. (1962). The genetics of Streptomyces coelicolor. Adv. Genet. 11, 273–342.Google Scholar
Hopwood, D. A., Sermonti, G. & Spada-Sermonti, I. (1963). Heterozygous clones in Streptomyces coelicolor. J. gen. Microbiol. 30, 249–260.Google Scholar
Jacob, F. & Wollman, E. L. (1958). Genetic and physical determinations of chromosomal segments in E. coli. Symp. Soc. exp. Biol. 12, 75–92.Google Scholar
Margolin, P. (1963). Genetic fine structure of the leucine operon in Salmonella. Genetics, 48, 441–457.CrossRefGoogle ScholarPubMed
Morse, M. L., Lederberg, E. M. & Lederberg, J. (1956). Transductional heterogenotes in Escherichia coli. Genetics, 41, 758–779.CrossRefGoogle ScholarPubMed
Nester, E. W., Schafer, M. & Lederberg, J. (1963). Gene linkage in DNA transfer: a cluster of genes concerned with aromatic biosynthesis in Bacillus subtilis. Genetics, 48, 529–551.CrossRefGoogle ScholarPubMed
Sanderson, K. E. & Demerec, M. (1964). Genetic map of Salmonella typhimurium. Microb. Genet. Bull. 20, 11–15 (personal communication).Google Scholar
Sermonti, G., Mancinelli, A. & Spada-Sermonti, I. (1960). Heterogeneous clones (hetero-clones) in Streptomyces coelicolor A3(2). Genetics, 45, 669–672.CrossRefGoogle Scholar
Sermonti, G. & Hopwood, D. A. (1964). Genetic recombination in Streptomyces. The Bacteria, Vol. V, pp. 223–251. New York: Academic Press.Google Scholar
Yoshikawa, H. & Sueoka, N. (1963). Sequential replication of Bacillus subtilis chromosome, I. Comparison of marker frequencies in exponential and stationary growth phases. Proc. natn. Acad. Sci., U.S.A. 49, 559–566.Google Scholar
Zinder, N. D. (1960). Hybrids of Escherichia and Salmonella. Science, N.Y. 131, 813–815.CrossRefGoogle ScholarPubMed