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A new allele of the Gpi-1t temporal gene that regulates the expression of glucose phosphate isomerase in mouse oocytes

Published online by Cambridge University Press:  14 April 2009

John D. West  and
Graham Fisher
John D. West
Affiliation:
MRC Radiobiology Unit, Harwell, Didcot, Oxon OX11 0RD, England
Graham Fisher
Affiliation:
MRC Radiobiology Unit, Harwell, Didcot, Oxon OX11 0RD, England
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The dimeric enzyme glucose phosphate isomerase (GPI-1) is regulated in oocytes by a cis-acting temporal gene (Gpi-1t) that maps close to the structural gene (Gpi-1s). Quantitative cellulose acetate electrophoresis of GPI-1 allozymes from unfertilized eggs produced by various Gpi-1sa / Gpi-1sb heterozygous females revealed a new Gpi-1t allele that we have designated Gpi-1tc. This allele is present in 101/H mice and a partially congenie stock that carries the Gpi-lsa gene derived from the AKR strain. We have confirmed that Gpi-1tc is closely linked to Gpi-1s and that it is cis-acting. It produces higher levels of GPI-1 in unfertilized eggs than the other two Gpi-lt alleles that are known (Gpi-1ta and Gpi-1tb) but has no effect on GPI-1 in somatic tissues or spermatozoa. This new Gpi-1t allele represents a third developmental programme for GPI-1 expression in oocytes.

Type
Research Article
Information
Genetics Research , Volume 44 , Issue 2 , October 1984 , pp. 169 - 181
Copyright
Copyright © Cambridge University Press 1984

References

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