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Mutations affecting cysteine synthesis in Aspergillus nidulans: characterization and chromosome mapping

Published online by Cambridge University Press:  14 April 2009

Jan Cybis ,
Renata Natorff ,
Irmina Lewandowska ,
Wieslawa Prazmo  and
Andrzej Paszewski
Jan Cybis
Affiliation:
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Rakowiecka 36, 02–532 Warsaw, Poland
Renata Natorff
Affiliation:
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Rakowiecka 36, 02–532 Warsaw, Poland
Irmina Lewandowska
Affiliation:
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Rakowiecka 36, 02–532 Warsaw, Poland
Wieslawa Prazmo
Affiliation:
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Rakowiecka 36, 02–532 Warsaw, Poland
Andrzej Paszewski*
Affiliation:
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Rakowiecka 36, 02–532 Warsaw, Poland
*
* Address correspondence to this author.

Summary

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Selection and mapping of mutations affecting cysteine synthesis in Aspergillus nidulans was carried out. A new locus, cysE, is described, the mutants of which are deficient in in vivo conversion of O-acetylserine to cysteine, a step mediated by cysteine synthase. Three loci (cysB, C and E) were thus found to control this step in vivo, apparently without affecting the enzyme activity in vitro. By scoring for propargylglycine sensitivity of cys mutants, chromosomal map positions were obtained for all five cysteine loci (A, B, C, D and E).

Type
Research Article
Information
Genetics Research , Volume 51 , Issue 2 , April 1988 , pp. 85 - 88
Copyright
Copyright © Cambridge University Press 1988

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