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Mutants of Escherichia coli sensitive to methylene blue and acridines

Published online by Cambridge University Press:  14 April 2009

Yoshinobu Sugino
Yoshinobu Sugino
Affiliation:
Department of Biology, School of Science, Osaka University, Japan
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A mutant (Mb-s) of Escherichia coli K12 which is more sensitive than wild-type (Mb-r) to such dyes as methylene blue or acridines was studied. The Mb-s mutant can also be disinfected of F factor with lower concentrations of acridine orange than Mb-r bacteria. The Mb locus was mapped by crosses between Hfr and F−, F-duction with F13, and transduction with phage P1. It was found to be situated between the pur and T6 loci on the chromosome. Moreover, it was found to be contained in the chromosomal fragment carried by the F-prime, F13. In a heterogenote obtained by F-duction, the Mb-r gene is dominant over the Mb-s gene. The Mb gene was transduced jointly with the pur gene by phage P1.

Type
Research Article
Information
Genetics Research , Volume 7 , Issue 1 , February 1966 , pp. 1 - 11
Copyright
Copyright © Cambridge University Press 1966

References

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