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Mutants of Coprinus lagopus selected for resistance to 2-deoxy-D-glucose

Published online by Cambridge University Press:  14 April 2009

David Moore  and
George R. Stewart
David Moore
Affiliation:
The Department of Botany, The University, Manchester, M13 9PL
George R. Stewart
Affiliation:
The Department of Botany, The University, Manchester, M13 9PL

Summary

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The glucose analogue 2-deoxy-D-glucose seriously inhibits the growth of Coprimts lagopus. Following treatment with N-methyl-N′-nitro-N-nitrosoguanidine 388 resistant mutants were isolated. It is shown that the mutants isolated are probably allelic; they were phenotypically similar and no complementation was observed. The mutants were pleiotropic in the sense that although they were initially selected only for resistance to 2-deoxy-D-glucose they were found to be cross-resistant to both of the related analogues, sorbose and glucosamine. Furthermore, the mutants were unable to utilize fructose as a sole carbon source. It is demonstrated that the inability to utilize fructose results from a defect in sugar transport. The gene symbol ftr is proposed for this cistron and it is shown that though the gene is quite closely linked to its own centromere, it is unlinked to centromere markers of the six known linkage groups.

Type
Research Article
Information
Genetics Research , Volume 18 , Issue 3 , December 1971 , pp. 341 - 352
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Barban, S. (1962). Studies on the mechanism of resistance to 2-deoxy-D-glucose in mammalian cell cultures. The Journal of Biological Chemistry 237, 291–295.CrossRefGoogle ScholarPubMed
Barban, S. (1963). Hormonal induced resistance to 2-deoxy-D-glucose in mammalian cell cultures. Federation Proceedings. Federation of American Societies f or Experimental Biology 22, 408 (abstract).Google Scholar
Elorza, M. V. & Arst, H. N. Jr., (1971). Sorbose resistant mutants of Aspergillus nidulans. Molecular and General Genetics 111, 186–193.CrossRefGoogle ScholarPubMed
Heredia, C. F. & Sols, A. (1964). Metabolic studies with 2-deoxyhexoses. II. Resistance to 2-deoxyglucose in a yeast mutant. Biochimica et biophysica acta 86, 224–228.CrossRefGoogle Scholar
Klingmuller, W. (1967 a). Die Aufnahme de Zucker Sorbose, Fructose und Glucose durch Sorbose-resistente Mutanten von Neurospora crossa. Zeitschrift für Naturforschung 22b, 327–335.CrossRefGoogle Scholar
Klingmüller, W. (1967 b). Kreuzungs-analyse sorbose-resistenter Mutanten von Neurospora crossa. Molecular and General Genetics 100, 109–116.CrossRefGoogle Scholar
Maitra, P. K. (1970). A glucokinase from Saccharomyces cerevisiae. The Journal of Biological Chemistry 245, 2423–2431.CrossRefGoogle ScholarPubMed
Megnet, R. (1965). Effect of 2-deoxyglucose on Schizosaccharomyces pombe. Journal of Bacteriology, 90, 1032–1035.CrossRefGoogle ScholarPubMed
Moore, D. (1966). New method of isolating the tetrads of agarics. Nature 209, 1157–1158.CrossRefGoogle Scholar
Moore, D. (1967). Four new linkage groups in Coprinus lagopus. Genetical Research 9, 331–342.Google Scholar
Moore, D. (1968). The effect of 2-deoxy-D-glucose on the growth and respiration of Coprinus lagopus. Journal of General Microbiology, 52, 433–439.CrossRefGoogle Scholar
Moore, D. (1969 a). Sources of carbon and energy used by Coprinus lagopus sensu Buller. Journal of General Microbiology 58, 49–56.CrossRefGoogle Scholar
Moore, D. (1969 b). The mutagenic action of N-methyl-N′-nitro-N-nitrosoguanidine on Coprinus lagopus. Journal of General Microbiology 55, 121–125.CrossRefGoogle ScholarPubMed
Moore, D. & Stewart, G. R. (1971). Hexose analogue inhibitions in Coprinus. II. Effects of 2-deoxy-D-glucose, D-glucosamine, and L-sorbose on the growth of hyphae. Journal of General Microbiology (in the Press).Google Scholar
Webb, J. L. (1966). Enzyme and Metabolic Inhibitors, vol. 2, p. 386. London: Academic Press.Google Scholar