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Mutants of bacteriophage lambda able to grow on the restricting host Escherichia coli strain W

Published online by Cambridge University Press:  14 April 2009

S. W. Glover  and
J. Aronovitch
S. W. Glover
Affiliation:
Medical Research Council, Microbial Genetics Research Unit, Hammersmith Hospital, Ducane Road, London, W.12
J. Aronovitch
Affiliation:
Medical Research Council, Microbial Genetics Research Unit, Hammersmith Hospital, Ducane Road, London, W.12
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Escherichia coli strain W adsorbs phage λ very efficiently but the phage does not form plaques on this strain because the DNA of λ is broken down in a majority of the infected cells shortly after adsorption. In a 10−3 to 10−4 fraction of the infected cells λ grows and small bursts of phage are produced. This phage does not carry the W-specific host modification and is unable to complete a second round of infection in W (Kerszman, Glover & Aronovitch, 1967). λω mutants have been isolated which are able to escape this restriction process and which plate on W with an efficiency of 1·0 and when grown in W these mutants carry a W-specific host modification.

Type
Short Papers
Information
Genetics Research , Volume 9 , Issue 1 , February 1967 , pp. 129 - 133
Copyright
Copyright © Cambridge University Press 1967

References

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