Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-27T02:08:39.907Z Has data issue: false hasContentIssue false
  • English
  • Français

Mutants in Saccharomyces lactis controlling both β-glucosidase and β-galactosidase activities*

Published online by Cambridge University Press:  14 April 2009

M. Tingle  and
H. O. Halvorson
M. Tingle
Affiliation:
Laboratory of Molecular Biology and Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706
H. O. Halvorson
Affiliation:
Laboratory of Molecular Biology and Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In Saccharomyces lactis, a class of mutants isolated for low β-glucosidase activity are reduced in activity for β-galactosidase as well. Genetic studies indicate that their properties are the result of a single mutation in a nuclear gene. In diploide containing a wild-type and mutant β-galactosidase allele, the mutant phenotype is partially dominant. The two enzymes can be separated physically and under appropriate conditions are induced independently in wild-type strains.

Type
Research Article
Information
Genetics Research , Volume 19 , Issue 1 , February 1972 , pp. 27 - 32
Copyright
Copyright © Cambridge University Press 1972

References

REFERENCES

Ames, B. N., Martin, R. G. & Garry, B. J. (1961). The first step of histidine biosynthesis. Journal of Biological Chemistry 236, 2019–2026.CrossRefGoogle ScholarPubMed
Duerksen, J. D. & Halvorson, H. O. (1958). Purification and properties of an inducible β-glucosidase in yeast. Journal of Biological Chemistry 233, 1113–1120.CrossRefGoogle Scholar
Fleming, L. W. & Duerksen, J. D. (1967). Evidence for multiple molecular forms of yeast β-glucosidase hi a hybrid yeast. Journal of Bacteriology 93, 142–150.CrossRefGoogle Scholar
Herman, A. I. (1963). Genetic control of β-glucosidase synthesis in yeast. Ph.D. thesis, University of Wisconsin.Google Scholar
Herman, A. & Halvorson, H. O. (1963 a). Identification of the structural gene for β-glucosidase in Saccharomyces lactis. Journal of Bacteriology 85, 895–900.CrossRefGoogle ScholarPubMed
Herman, A. & Halvorson, H. O. (1963 b). Genetic control of β-glucosidase synthesis in Saccharomyces lactis. Journal of Bacteriology 85, 901–910.CrossRefGoogle ScholarPubMed
McQuillan, A. M. & Halvorson, H. O. (1962). Metabolic control of β-glucosidase synthesis in yeast. Journal of Bacteriology 84, 23–30.CrossRefGoogle Scholar
Tingle, M. (1967). Biochemical and genetic studies in Saccharomyces lactis. Ph.D. thesis, University of Wisconsin.Google Scholar
Tingle, M., Herman, A. & Halvorson, H. O. (1968). Characterization and mapping of histidine genes in Saccharomyces lactis. Genetics 58, 361–371.CrossRefGoogle ScholarPubMed
Van Der Walt, J. P. (1965). The emendation of the genus Kluyveromyces. Antonie Van Leewenhoek: Journal of Microbiology and Serology 31, 345–348.Google ScholarPubMed