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Mutant Hfr strains defective in transfer: restoration by F-like and I-like de-repressed R factors

Published online by Cambridge University Press:  14 April 2009

Marylyn Cooke ,
Elinor Meynell  and
A. M. Lawn
Marylyn Cooke
Affiliation:
M.R.C. Microbial Genetics Research Unit, London, W. 12, and Guinness–Lister Research Unit and Departments of Microbiology and Electron Microscopy, Lister Institute, Chelsea Bridge Road, London, S.W.1*
Elinor Meynell
Affiliation:
M.R.C. Microbial Genetics Research Unit, London, W. 12, and Guinness–Lister Research Unit and Departments of Microbiology and Electron Microscopy, Lister Institute, Chelsea Bridge Road, London, S.W.1*
A. M. Lawn
Affiliation:
M.R.C. Microbial Genetics Research Unit, London, W. 12, and Guinness–Lister Research Unit and Departments of Microbiology and Electron Microscopy, Lister Institute, Chelsea Bridge Road, London, S.W.1*
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Mutants of Hfr strains of Escherichia coli K12 defective in conjugation owing to failure to produce sex pili were isolated by resistance to F-specific phage. Tests of the ability of six de-repressed F-like R factors and four de-repressed I-like R factors to restore Hfr donor behaviour to these mutants indicated that there were several ways in which such restoration could occur. Of a total of 26 defective mutants, 12 were restored by F-like R factors. In these twelve, the function of the integrated F factor was evidently restored, because the sex pili contained F pilin subunits, distinguishable from the R pilin by serological tests. In contrast, among the four I-like R factors, only two were effective, butin all 26 defective mutants; the restored Hfr bacteria produced only I-like pili. The I-like sex factors, in restoring Hfr donor behaviour, did not therefore act by complementing the defective F.

Type
Research Article
Information
Genetics Research , Volume 16 , Issue 1 , August 1970 , pp. 101 - 112
Copyright
Copyright © Cambridge University Press 1970

References

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