Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-25T06:53:15.358Z Has data issue: false hasContentIssue false
  • English
  • Français

Mutant alleles of the meiotic locus, mei-9, differ in degree of effects on rod chromosome magnification and ring chromosome transmission in Drosophila

Published online by Cambridge University Press:  14 April 2009

Donald J. Komma ,
Heath Graves  and
Sharyn A. Endow
Donald J. Komma
Affiliation:
Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina27710
Heath Graves
Affiliation:
Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina27710
Sharyn A. Endow*
Affiliation:
Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina27710
*
* Corresponding author.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Two mutant alleles of the meiotic locus, mei-9, have been examined for their effect on magnification of a rod Xbb chromosome and transmission of a ring Xbb chromosome under magnifying conditions. Our results indicate that the effects of these two mutations are allele-specific: mei-9a strongly inhibits both rod chromosome magnification and ring chromosome loss under magnifying conditions, while mei-9b has a smaller inhibitory effect on rod chromosome magnification and on the transmission of ring chromosomes under magnifying conditions. These observations can be explained by a difference in leakiness between the two alleles. Our results demonstrate that mutants defective in excision repair and repair replication inhibit ribosomal gene magnification. This suggests that a component of the excision repair pathway is involved in the process of magnification.

Type
Research Article
Information
Genetics Research , Volume 53 , Issue 3 , June 1989 , pp. 155 - 161
Copyright
Copyright © Cambridge University Press 1989

References

Atwood, K. C. (1969). Some aspects of the bobbed problem in Drosophila. Genetic Supplement 61, 319–327.Google ScholarPubMed
Baker, B. S. & Carpenter, A. T. C. (1972). Genetic analysis of sex chromosomal meiotic mutants in Drosophila melanogaster. Genetics 71, 255–286.CrossRefGoogle Scholar
Boncinelli, E., Graziani, F., Polito, L., Malva, C. & Ritossa, F. (1972). rDNA magnification at the bobbed locus of the Y chromosome in Drosophila melanogaster. Cell Differentiation 1, 133–142.CrossRefGoogle Scholar
Boyd, J. B., Golino, M. D., Nguyen, T. D. & Green, M. M. (1976 a). Isolation and characterization of X-linked mutants of Drosophila melanogaster which are sensitive to mutagens. Genetics 84, 485–506.CrossRefGoogle ScholarPubMed
Boyd, J. B., Golino, M. D. & Setlow, R. B. (1976 b). The mei-9a mutant of Drosophila melanogaster increases mutagen sensitivity and decreases excision repair. Genetics 84, 527–544.Google Scholar
Boyd, J. B. & Setlow, R. B. (1976). Characterization of postreplication repair in mutagen-sensitive strains of Drosophila melanogaster. Genetics 84, 507–526.CrossRefGoogle ScholarPubMed
Boyd, J. B. & Shaw, K. E. S. (1982). Postreplication repair defects in mutants of Drosophila melanogaster. Molecular and General Genetics 186, 289–294.CrossRefGoogle ScholarPubMed
Endow, S. A. & Komma, D. J. (1986). One-step and stepwise magnification of a bobbed lethal chromosome in Drosophila melanogaster. Genetics 114, 511–523.CrossRefGoogle ScholarPubMed
Endow, S. A., Komma, D. J. & Atwood, K. C. (1984). Ring chromosomes and rDNA magnification in Drosophila. Genetics 108, 969–983.CrossRefGoogle ScholarPubMed
Hawley, R. S., Marcus, C. H., Cameron, M. L., Schwartz, R. L. & Zitron, A. R. (1985). Repair-defect mutations inhibit rDNA magnification in Drosophila and discriminate between meiotic and premeiotic magnification. Proceedings of the National Academy of Sciences, USA 82, 8095–8099.CrossRefGoogle ScholarPubMed
Hawley, R. S. & Tartof, K. D. (1983). The effect of mei-41 on rDNA redundancy in Drosophila melanogaster. Genetics 104, 63–80.CrossRefGoogle ScholarPubMed
Henderson, A. & Ritossa, F. (1970). On the inheritance of rDNA of magnified bobbed loci in D. melanogaster. Genetics 66, 463–473.Google Scholar
Komma, D. J. & Endow, S. A. (1986). Magnification of the ribosomal genes in female Drosophila melanogaster. Genetics 114, 859–874.CrossRefGoogle ScholarPubMed
Lindsley, D. L. & Grell, E. H. (1968). Genetic variations of Drosophila melanogaster. Carnegie Institution of Washington Publication 627.Google Scholar
Lindsley, D. & Zimm, G. (1985). The genome of Drosophila melanogaster. Part 1: Genes A–K. Drosophila Information Service 62.Google Scholar
Locker, D. (1976). Instability at the bobbed locus following magnification in Drosophila melanogaster. Molecular and General Genetics 143, 261–268.Google Scholar
Nguyen, T. D. & Boyd, J. B. (1977). The meiotic-9 (mei-9) mutants of Drosophila melanogaster are deficient in repair replication of DNA. Molecular and General Genetics 158, 141–147.CrossRefGoogle Scholar
Nguyen, T. D., Green, M. M. & Boyd, J. B. (1978). Isolation of two X-linked mutants in Drosophila melanogaster which are sensitive to γ-rays. Mutation Research 49, 139–143.CrossRefGoogle ScholarPubMed
Polito, L. C., Cavaliere, D., Zazo, A. & Furia, M. (1982). A study of rDNA magnification phenomenon in a repair-recombination deficient mutant of Drosophila melanogaster. Genetics 102, 39–48.CrossRefGoogle Scholar
Ritossa, F. M. (1968). Unstable redundancy of genes for ribosomal RNA. Proceedings of the National Academy of Sciences, USA 60, 509–516.CrossRefGoogle ScholarPubMed
Ritossa, F. (1973). Crossing-over between X and Y chromosomes during ribosomal DNA magnification in Drosophila melanogaster. Proceedings of the National Academy of Sciences, USA 70, 1950–1954.CrossRefGoogle ScholarPubMed
Ritossa, F., Malva, C., Boncinelli, E., Graziani, F. & Polito, L. (1971). The first steps of magnification of DNA complementary to ribosomal RNA in Drosophila melanogaster. Proceedings of the National Academy of Sciences, USA 68, 1580–1584.CrossRefGoogle Scholar
Ritossa, F. M. & Scala, G. (1969). Equilibrium variations in the redundancy of rDNA in Drosophila melanogaster. Genetics Supplement 61, 305–317.Google ScholarPubMed
Smith, P. D. (1973). Mutagen sensitivity of Drosophila melanogaster. I. Isolation and preliminary characterization of a methyl methanesulphonate-sensitive strain. Mutation Research 20, 215–220.CrossRefGoogle ScholarPubMed
Smith, P. D. (1976). Mutagen sensitivity of Drosophila melanogaster. III. X-linked loci governing sensitivity to methyl methanesulfonate. Molecular and General Genetics 149, 73–85.CrossRefGoogle ScholarPubMed
Stern, C. (1929). Über die additive Wirkung multipler Allele. Biologisches Zentralblatt 49, 261–290.Google Scholar
Tartof, K. D. (1974). Unequal mitotic sister chromatid exchange as the mechanism of ribosomal RNA gene magnification. Proceedings of the National Academy of Sciences, USA 71, 1272–1276.Google Scholar