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Mouse testicular protein variants, including tubulin, detected by isoelectrofocusing two-dimensional gel electrophoresis

Published online by Cambridge University Press:  14 April 2009

J. Schneider ,
R. P. Erickson  and
S. Hanash
J. Schneider
Affiliation:
Departments of Human Genetics and Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan 48109–0618
R. P. Erickson*
Affiliation:
Departments of Human Genetics and Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan 48109–0618
S. Hanash
Affiliation:
Departments of Human Genetics and Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan 48109–0618
*
* To whom correspondence should be addressed.
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We have utilized isoelectrofocusing two-dimensional polyacrylamide gel electrophoresis (IEF-ID-PAGE) to determine the frequency of variant proteins detected in mouse testes, and to examine an inbred strain with spermatogenic abnormalities. The observed frequency of variants, 4·2 ± 1·0%, is comparable to results reported for liver. A variant for a protein, identified as tubulin by immunoblotting, was observed in an inbred strain (PL/J) which has defective spermatogenesis, but was also observed in another inbred strain without spermatogenic defects. The gene symbol Tbn is proposed for this β-tubulin locus.

Type
Research Article
Information
Genetics Research , Volume 50 , Issue 3 , December 1987 , pp. 225 - 233
Copyright
Copyright © Cambridge University Press 1987

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