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Mitotic mutants of Aspergillus nidulans

Published online by Cambridge University Press:  14 April 2009

N. Ronald Morris
N. Ronald Morris
Affiliation:
Department of Pharmacology, C.M.D.N.J. – Rutgers Medical School, Piscataway, New Jersey 08854, U.S.A.
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Forty-five temperature-sensitive mutants of Aspergillus nidulans which are defective in nuclear division, septation or distribution of nuclei along the mycelium have been isolated, and most have been subjected to complementation analysis and mapped to chromosome. Thirty-five of the mutants were unable to complete nuclear division at the restrictive temperature. Twenty-six of these mutants exhibited a co-ordinate drop in both spindle and chromosome mitotic indices at 42 °C, indicating that they fail to enter mitosis. These mutants have been assigned to the gene symbol nim. Nine mutants exhibited a co-ordinate rise in spindle and chromosome mitotic indices at 42 °C, indicating that they are arrested in mitosis. These mutants were assigned the gene symbol bim. Five mutants failed to form septa and were given the gene symbol sep; and five mutants had an abnormal nuclear distribution and were given the gene symbol nud. All of the mutations were recessive. Most of the mutants were in different complementation groups. Mutants in the same complementation groups were phenotypically similar, but phenotypically similar mutants were not necessarily or usually in the same complementation group. There was no evidence for genetic clustering of phenotypically similar mutants. The mutants were located on all eight chromosomes.

Type
Research Article
Information
Genetics Research , Volume 26 , Issue 3 , December 1975 , pp. 237 - 254
Copyright
Copyright © Cambridge University Press 1975

References

REFERENCES

Bainbridge, B. W. (1971). Macromolecular composition and nuclear division during spore germination in Aspergillus nidulans. Journal of General Microbiology 66, 319–325.CrossRefGoogle ScholarPubMed
Byers, B. & Goetsch, L. (1973). Duplication of spindle plaques and integration of the yeast cell cycle. Cold Spring Harbor Symposia on Quantitative Biology 38, 123–131.Google Scholar
Clutterbuck, A. J. (1970). Synchronous nuclear division and septation in Aspergillus nidulans. Journal of General Microbiology 60, 133–135.Google Scholar
Clutterbuck, A. J. (1973). Gene symbols in Aspergillus nidulans. Genetical Research 21, 291–296.Google Scholar
Elliot, C. G. (1960). The cytology of Aspergillus nidulans. Genetical Research 1, 462–476.Google Scholar
Forbes, E. (1959). Use of mitotic segregation for assigning genes to linkage groups in Aspergillus nidulans. Heredity 13, 67–80.CrossRefGoogle Scholar
Hartwell, L. H., Culotti, J. & Reid, B. (1970). Genetic control of the cell division cycle in yeast. 1. Detection of mutants. Proceedings of the National Academy of Science, U.S.A. 66, 352–359.Google Scholar
Hartwell, L. H., Mortimer, R. K., Culotti, J. & Culotti, M. (1973). Genetic control of the cell division cycle in yeast. V. Genetic analysis of cdc mutants. Genetics 74, 267–286.CrossRefGoogle ScholarPubMed
Hastie, A. C. (1970). Benlate-induced instability of Aspergillus diploids. Nature 226, 771.CrossRefGoogle ScholarPubMed
Howell, S. H. & Naliboff, J. A. (1973). Conditional mutants of Chlamydomonas reinhardi blocked in the vegetative cell cycle. 1. An analysis of cell cycle block points. Journal of Cell Biology 57, 760–772.Google Scholar
Kafer, E. (1958). An 8-chromosome map of Aspergillus nidulans. Advances in Genetics 9, 105–145.Google Scholar
Kessel, M. & Rosenberger, R. F. (1968). Regulation and timing of deoxyribonucleic acid synthesis in hyphae of Aspergillus nidulans. Journal of Bacteriology 95, 2275–2281.CrossRefGoogle ScholarPubMed
McCully, K. S. & Forbes, E. (1965). The use of p-fluorophenylalanine with ‘master strains’ for assigning genes to linkage groups. Genetical Research 6, 352–359.CrossRefGoogle ScholarPubMed
Mazia, D. & Dan, K. (1952). The isolation and characterisation of the mitotic apparatus of dividing cells. Proceedings of the National Academy of Science, U.S.A. 38, 826–911.CrossRefGoogle ScholarPubMed
Mitchison, J. M. (1971). The Biology of the Cell Cycle. Cambridge University Press.Google Scholar
Moens, P. B. & Rapport, E. (1971). Spindles, spindle plaques and meiosis in the yeast Saccharomyces cerevisiae. Journal of Cell Biology 50, 344–361.Google Scholar
Morpurgo, G. (1961). Somatic segregation induced by p-fluorophenylalanine. Aspergillus Newsletter 2, 10.Google Scholar
Ogata, W. N. (1962). Preservation of Neurospora stock cultures with anhydrous silica gel. Neurospora Newsletter 1, 13.Google Scholar
Pontecorvo, G. & Kafer, E. (1958). Genetic analysis based on mitotic recombination. Advances in Genetics 9, 71–104.CrossRefGoogle ScholarPubMed
Pontecorvo, G., Roper, J. A., Hemmons, L. M., Macdonald, K. D., Bufton, A. W. J. (1953). The genetics of Aspergillus nidulans. Advances in Genetics 5, 141–238.Google Scholar
Robinow, C. F. & Caten, C. E. (1969). Mitotis in Aspergillus nidulans. Journal of Cell Science 5, 403–437.Google Scholar
Robinow, C. F. & Marak, J. (1966). A fiber apparatus in the nucleus of the yeast cell. Journal of Cell Biology 29, 129–151.Google Scholar
Rosenberger, R. F. & Kessel, M. (1967). Synchrony of nuclear replication in individual hyphae of Aspergillus nidulans. Journal of Bacteriology 94, 1464–1469.CrossRefGoogle ScholarPubMed