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Microsatellite polymorphisms in a wild population of Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

Phillip R. England ,
David A. Briscoe  and
Richard Frankham
Phillip R. England*
Affiliation:
School of Biological Sciences, Macquarie University, N.S.W. 2109, Australia
David A. Briscoe
Affiliation:
School of Biological Sciences, Macquarie University, N.S.W. 2109, Australia
Richard Frankham
Affiliation:
School of Biological Sciences, Macquarie University, N.S.W. 2109, Australia
*
*Phillip R. England at above address. Phone: (612) 850 8187; Fax: (612) 850 8245; E-mail: [email protected]
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Summary

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Highly variable DNA polymorphisms called microsatellites are rapidly becoming the marker of choice in population genetic studies. Until now, microsatellites have not been utilized for Drosophila studies. We have identified eight polymorphic microsatellite loci in Drosophila melanogaster and used them to characterize the genetic variation in a wild population from the Tyrrell's winery in Australia. Microsatellites were isolated from a partial genomic DNA library. All microsatellites consist of (AC)n repeats ranging from n = 2 to n = 24. Six loci were assigned to chromosomal location by genetic mapping, with three loci on chromosome II, one locus on chromosome III and two loci on the X chromosome. Up to four microsatellite loci were multiplexed in the same reaction. Microsatellite variation is substantially greater than allozyme variation in the Tyrrell's Drosophila population. 80% of the microsatellite loci examined are polymorphic, compared with 28% of allozymes. The mean number of alleles per polymorphic locus is 5·2 in microsatellites compared with 30 in allozymes. The average observed heterozygosity of polymorphic microsatellites is 47% compared with 26% for allozymes. Microsatellite variation in Drosophila melanogaster is similar to that reported for other insects. Higher variability commends microsatellites over allozymes for genetic studies in Drosophila melanogaster.

Type
Short Paper
Information
Genetics Research , Volume 67 , Issue 3 , June 1996 , pp. 285 - 290
Copyright
Copyright © Cambridge University Press 1996

References

Bruford, M. W., & Wayne, R. K., (1993). Microsatellites and their application to population genetic studies. Current Opinion in Genetics and Development 3, 939–943.CrossRefGoogle ScholarPubMed
Choudhary, M., Strassmann, J. E., Solis, C. R., & Queller, D. C., (1993). Microsatellite variation in a social insect. Biochemical Genetics 31, 87–96.Google Scholar
Estoup, A., Solignac, M., Harry, M., & Cornuet, J.-M., (1993). Characterisation of (GT)n and (CT)n microsatellites in two insect species: Apis melifera and Bombus terrestris. Nucleic Acids Research 21, 1427–1431.CrossRefGoogle Scholar
Gertsch, P., Pamilo, P., & Varvio, S.-L., (1995). Microsatellites reveal high genetic diversity within colonies of Camponotus ants. Molecular Ecology 4, 257–260.CrossRefGoogle ScholarPubMed
Hamaguchi, K., Ito, Y., & Takenaka, O., (1993). Dinucleotide repeat polymorphisms in a polygynous ant, Leptothorax spinosior, and their use for measurement of relatedness. Naturwissenschaften 80, 179–181.Google Scholar
Hughes, C. R., & Queller, D. C., (1993). Detection of highly polymorphic microsatellite loci in a species with little allozyme polymorphism. Molecular Ecology 2, 131–137.Google Scholar
Lanzaro, G. C., Zheng, L., Toure, Y. T., Traore, S. F., Kafatos, F. C., & Vernick, K. D., (1995). Microsatellite DNA and isozyme variability in a West African population of Anopheles gambiae. Insect Molecular Biology 4, 105–112.CrossRefGoogle Scholar
Lincoln, S. E., Daly, M. J., & Lander, E. S., (1991). Primer: a Computer Program for Automatically Selecting PCR Primers, Version 0.5. Massachusetts, USA: MIT Centre for Genome Research and Whitehead Institute for Biomedical Research.Google Scholar
Lindsley, D. L., & Zimm, G. G., (1992). The genome of Drosophila melanogaster. San Diego, CA: Academic Press.Google Scholar
Litt, M., & Luty, J. A., (1989). A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. American Journal of Human Genetics 44, 397–401.Google Scholar
Miller, S. A., Dykes, D. D., & Polesky, H. F., (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research 16, 1215.CrossRefGoogle ScholarPubMed
Pardue, M. L., Lowenhaupt, K., Rich, A., & Nordheim, A., (1987). (dC-dA)n. (dG-dT)n sequences have evolutionarily conserved chromosomal locations in Drosophila.with implications for roles in chromosome structure and function. EM BO Journal 6, 1781–1789.Google ScholarPubMed
Rassmann, K., Schlotterer, C., & Tautz, D., (1991). Isolation of simple-sequence loci for use in polymerase chain reaction-based DNA fingerprinting. Electrophoresis 12, 113–118.Google Scholar
Swofford, D. L., & Selander, R. B., (1981). BIOSYS-1: A FORTRAN program for the comprehensive analysis of electrophoretic data in population genetics and systematics. Journal of Heredity 72, 281–283.Google Scholar
Tautz, D., (1989). Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucleic Acids Research 17, 6463–6471.Google Scholar
Tautz, D., & Renz, M., (1984). Simple sequences are ubiquitous repetitive components of eukaryotic genomes. Nucleic Acids Research 10, 4127–4138.CrossRefGoogle Scholar
Traut, W., Epplen, J. T., Weichenhan, D., & Rohwedel, J., (1992). Inheritance and mutation of hypervariable (GATA)n microsatellite loci in a moth, Ephestia kuehniella. Genome 35, 659–666.Google Scholar
Weber, J. L., (1991). Human DNA polymorphisms based on length variations in simple-sequence tandem repeats. In Genome Analysis: A Practical Approach, Volume 1 (ed. Davies, K. E., & Tilghman, S. M.), pp. 160–181. New York: Cold Spring Harbor Laboratory Press.Google Scholar
Weber, J. L., & May, P. E., (1989). Abundant class of human polymorphism which can be typed using the polymerase chain reaction. American Journal of Human Genetics 44, 388–396.Google Scholar
Zheng, L., Collins, F. H., Kumar, V., & Kafatos, F. C., (1993). A detailed genetic map for the X chromosome of the malaria vector, Anopheles gambiae. Science 261, 605–608.CrossRefGoogle ScholarPubMed