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A method for selecting streptomycin-sensitive mutants of R factors which adenylate streptomycin*

Published online by Cambridge University Press:  14 April 2009

J. H. Harwood ,
J. Janjigian  and
D. H. Smith
J. H. Harwood
Affiliation:
Departments of Bacteriology and Immunology and Pediatrics, Harvard Medical School, and Children's Hospital Medical Center, Boston, Mass. 02115, U.S.A.
J. Janjigian
Affiliation:
Departments of Bacteriology and Immunology and Pediatrics, Harvard Medical School, and Children's Hospital Medical Center, Boston, Mass. 02115, U.S.A.
D. H. Smith
Affiliation:
Departments of Bacteriology and Immunology and Pediatrics, Harvard Medical School, and Children's Hospital Medical Center, Boston, Mass. 02115, U.S.A.
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An Arg− conditionally streptomycin (Sm)-dependent strain of E. coli B that carries an R factor mediating Sm adenylation cannot grow on minimal agar containing 20 μg Sm/ml unless the strain either becomes Arg+ or loses the ability to adenylate Sm (Harwood & Smith, 1969b). We have therefore studied the efficacy of this selection with respect to enriching for SmS mutants of such R factors. Following u.v. mutagenesis, cells were plated on the selective medium; of the clones which were examined, Arg− R-SmS colonies were found up to 3000 times more frequently than Arg+ R-SmR colonies.

Type
Short Papers
Information
Genetics Research , Volume 14 , Issue 3 , December 1969 , pp. 323 - 327
Copyright
Copyright © Cambridge University Press 1969

References

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