Hostname: page-component-7bb8b95d7b-lvwk9 Total loading time: 0 Render date: 2024-09-12T11:06:11.805Z Has data issue: false hasContentIssue false
  • English
  • Français

A method for selecting streptomycin-sensitive mutants of R factors which adenylate streptomycin*

Published online by Cambridge University Press:  14 April 2009

J. H. Harwood ,
J. Janjigian  and
D. H. Smith
J. H. Harwood
Affiliation:
Departments of Bacteriology and Immunology and Pediatrics, Harvard Medical School, and Children's Hospital Medical Center, Boston, Mass. 02115, U.S.A.
J. Janjigian
Affiliation:
Departments of Bacteriology and Immunology and Pediatrics, Harvard Medical School, and Children's Hospital Medical Center, Boston, Mass. 02115, U.S.A.
D. H. Smith
Affiliation:
Departments of Bacteriology and Immunology and Pediatrics, Harvard Medical School, and Children's Hospital Medical Center, Boston, Mass. 02115, U.S.A.

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

An Arg− conditionally streptomycin (Sm)-dependent strain of E. coli B that carries an R factor mediating Sm adenylation cannot grow on minimal agar containing 20 μg Sm/ml unless the strain either becomes Arg+ or loses the ability to adenylate Sm (Harwood & Smith, 1969b). We have therefore studied the efficacy of this selection with respect to enriching for SmS mutants of such R factors. Following u.v. mutagenesis, cells were plated on the selective medium; of the clones which were examined, Arg− R-SmS colonies were found up to 3000 times more frequently than Arg+ R-SmR colonies.

Type
Short Papers
Information
Genetics Research , Volume 14 , Issue 3 , December 1969 , pp. 323 - 327
Copyright
Copyright © Cambridge University Press 1969

References

REFERENCES

Davis, B. D. (1948). Isolation of biochemically deficient mutants of bacteria by penicillin. J. Am. Chem. Soc. 70, 4267.CrossRefGoogle ScholarPubMed
Davis, B. D. & Mingioli, E. S. (1950). Mutants of Escherichia coli requiring methionine or vitamin B12. J. Bact. 60, 17.CrossRefGoogle ScholarPubMed
Gorini, L. & Kataja, E. (1964). Phenotypic repair by streptomycin of defective genotypes in E. coli. Proc. natn. Acad. Sci. U.S.A. 51, 487.CrossRefGoogle ScholarPubMed
Harwood, J. H. & Smith, D. H. (1969 a). Resistance factor-mediated streptomycin resistance. J. Bact. 97, 1262.CrossRefGoogle ScholarPubMed
Harwood, J. H. & Smith, D. H. (1969 b). Phenotypic suppression by streptomycin-adenylate and bluensomycin-adenylate. Genet. Res., Camb. 14, 259–273.CrossRefGoogle ScholarPubMed
Jacob, F. & Wollman, E. L. (1955). Étude génétique d'un bactériophage tempéré d'Escherichia coli: Effet du rayonnement ultraviolet sur la recombinaison génétique. Ann. inst. Pasteur 88, 724.Google Scholar
Kondo, S., Okanishi, M., Utahara, R., Maeda, K. & Umezawa, H. (1968). Isolation of kanamycin and paromamine inactivated by E. coli carrying R factor. J. Antibiot. (Tokyo), A 21 (1), 22.CrossRefGoogle ScholarPubMed
Smith, D. H. (1969). R factor-mediated resistance to aminoglycoside antibiotics. J. Infect. Dis. 119, 378CrossRefGoogle Scholar
Walton, J. R. & Smith, D. H. (1969). New hemolysin (γ) produced by Escherichia coli. J. Bact. 98, 304.CrossRefGoogle ScholarPubMed
Watanabe, T. (1964). Selected methods of genetic study of episome-mediated drug resistance in bacteria. In Meth. med. Res. 10, 202.Google ScholarPubMed
Watanabe, T. & Fukasawa, T. (1961). Episome-mediated transfer of drug resistance in Enterobacteriaceae. III. Transduction of resistance factors. J. Bact. 82, 202.CrossRefGoogle ScholarPubMed
Watanabe, T. & Lyang, K. W. (1962). Episome-mediated transfer of drug resistance in Enterobacteriaceae. V. Spontaneous segregation and recombination of resistance factors in Salmonella typhimurium. J. Bact. 84, 422.CrossRefGoogle ScholarPubMed