Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-27T01:15:01.042Z Has data issue: false hasContentIssue false
  • English
  • Français

Mapping and biochemical analysis of Hor 4 (Hrd G), a second locus encoding B hordein seed proteins in barley (Hordeum vulgare L.)

Published online by Cambridge University Press:  14 April 2009

Peter R. Shewry ,
Saroj Parmar ,
Julian Franklin  and
Rodger White
Peter R. Shewry*
Affiliation:
AFRC Institute of Arable Crops Research, Department of Biochemistry, Rothamsted Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
Saroj Parmar
Affiliation:
AFRC Institute of Arable Crops Research, Department of Biochemistry, Rothamsted Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
Julian Franklin
Affiliation:
AFRC Institute of Arable Crops Research, Department of Biochemistry, Rothamsted Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
Rodger White
Affiliation:
Department of Statistics, Rothamsted Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
*
* Corresponding author
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The hordein polypeptide controlled by the Hrd G (Hor 4) locus in Elgina and derived lines was purified by preparative isoelectric focusing. The amino acid composition was similar to those of the major B hordeins encoded by the Hor 2 locus. Genetic analysis confirmed that Hor 4 is located proximally to Hor 1 on the short arm of chromosome 5. It is speculated that Hor 4 arose by translocation of genes from Hor 2, possibly in an ancestor of Elgina.

Type
Research Article
Information
Genetics Research , Volume 51 , Issue 1 , February 1988 , pp. 5 - 12
Copyright
Copyright © Cambridge University Press 1988

References

Blake, T. K., Ullrich, S. E. & Nilan, R. A. (1982). Mapping of the Hor 3 locus encoding ‘D’ hordeins in barley. Theoretical and Applied Genetics 63, 367–371.Google Scholar
Brandt, A., Ingversen, J., Cameron-Mills, V., Schmitt, J. M. & Rasmussen, S. K. (1981). Molecular aspects of storage protein synthesis in barley endosperms. In Barley Genetics, vol. IV. Edinburgh: University of Edinburgh Press.Google Scholar
Bunce, N., White, R. P. & Shewry, P. R. (1985). Variation in estimates of molecular weights of cereal prolamins by SDS–PAGE. Journal of Cereal Science 3, 131–142.CrossRefGoogle Scholar
Bunce, N. A. C., Forde, B. G., Kreis, M. & Shewry, P. R. (1986). DNA restriction fragment length polymorphism at hordein loci: application to identifying and finger-printing barley cultivars. Seed Science and Technology 14, 419–429.Google Scholar
Cornish-Bowden, A. (1981). Interpretation of amino acid compositions. Trends in Biochenical Sciences 6, 217–219.Google Scholar
Doll, H. & Brown, A. D. H. (1979) Hordein variation in wild (Hordeum spontaneum) and cultivated (H. vulgare) barley. Canadian Journal of Genetics and Cytology 21, 391–404.CrossRefGoogle Scholar
Faulks, A. J., Shewry, P. R. & Miflin, B. J. (1981). The polymorphism and structural homology of storage polypeptides (hordein) coded by the Hor 2 locus in barley (Hordeum vulgare L.). Biochemical Genetics 19, 841–858.Google Scholar
Field, J. M., Shewry, P. R., Miflin, B. J. & March, J. F. (1982). The purification and characterization of homologous high molecular weight storage proteins from grain of wheat, rye and barley. Theoretical and Applied Genetics 62, 329–336.Google Scholar
Forde, B. G., Kreis, M., Bahramian, M. B., Matthews, J. A., Miflin, B. J., Thompson, R. D., Bartels, D. & Flavell, R. B. (1981). Molecular cloning and analysis of cDNA sequences derived from poly A+ RNA from barley endosperm: identification of B hordein related clones. Nucleic Acids Research 9, 6689–6707.CrossRefGoogle ScholarPubMed
Forde, J. (1985). The molecular biology of the high molecular weight glutenin subunits of wheat. Ph.D. Thesis, CNAA, U.K.Google Scholar
Friedman, M., Krull, L. H. & Cavins, J. F. (1970). The chromatographic determination of cystine and cysteine residues as S-β-(4-pyridylethyl) cysteine. Journal of Biological Chemistry 245, 3868–3871.Google Scholar
Jensen, J. & Jorgensen, J. H. (1975). The barley chromosome 5 linkage map. 1. Literature survey and map estimation procedure. Hereditas 80, 5–16.Google Scholar
Jensen, J., Jorgensen, J. H., Jenson, H. P., Giese, H. & Doll, H. (1980). Linkage of the hordein loci Hor 1 and Hor 2 with the powdery mildew resistance loci M1-k and M1-a on barley chromosome 5. Theoretical and Applied Genetics 58, 27–31.Google Scholar
Jensen, J. (1986). Co-ordinator's Report. Chromosome 5. Barley Genetics Newsletter 16, 32–34.Google Scholar
Johansen, H. B. & Shewry, P. R. (1986). Recommended designations for hordein alleles. Barley Genetics Newsletter 16, 9–11.Google Scholar
Kreis, M., Shewry, P. R., Forde, B. G., Rahman, S. & Miflin, B. J. (1983). Molecular analysis of a mutation conferring the high lysine phenotype on the grain of barley (Hordeum vulgare). Cell 34, 161–167.CrossRefGoogle ScholarPubMed
Kreis, M., Williamson, M. S., Forde, J., Clark, J., Buxton, B., Pywell, J., Marris, C., Gallois, P. & Shewry, P. R. (1987). Structure and regulation of seed protein genes in barley. Plant Physiology and Biochemistry 25, 291–302.Google Scholar
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 681–684.CrossRefGoogle ScholarPubMed
Netsvetaev, V. P. & Sozinov, A. A. (1982). Linkage studies of genes Glel and HrdF in barley chromosome 5. Barley Genetics Newsletter 12, 13–18.Google Scholar
Netsvetaev, V. P. & Sozinov, A. A. (1984). Location of a hordein G locus, HrdG, on chromosome 5 of barley. Barley Genetic Newsletter 14, 4–6.Google Scholar
Oram, R. N., Doll, H. & Køie, B. (1975). Genetics of two storage protein variants in barley. Hereditas 80, 53–58.Google Scholar
Rahman, S., Shewry, P. R. & Miflin, B. J. (1982). Differential protein accumulation during barley grain development. Journal of Experimental Botany 33, 717–728.Google Scholar
Shewry, P. R., Pratt, H. M., Finch, R. A. & Miflin, B. J. (1978). Genetic anaysis of hordein polypeptides from single seeds of barley. Heredity 40, 463–466.CrossRefGoogle Scholar
Shewry, P. R., Field, J. M., Kirkman, M. A., Faulks, A. J. & Miflin, B. J. (1980 a). The extraction, solubility and characterization of two groups of barley storage polypeptides. Journal of Experimental Botany 31, 393–407.Google Scholar
Shewry, P. R., Faulks, A. J., Pickering, R. A., Jones, I. T., Finch, R. A. & Miflin, B. J. (1980 b). The genetic analysis of barley storage proteins. Heredity 44, 383–389.Google Scholar
Shewry, P. R., Lew, E. J.-L. & Kasarda, D. D. (1981). Structural homology of storage proteins coded by the Hor 1 locus of barley (Hordeum vulgare L.). Planta 153, 246–253.CrossRefGoogle Scholar
Shewry, P. R., Finch, R., Parmar, S., Franklin, J. & Miflin, B. J. (1983). Chromosomal location of Hor 3, a new locus governing storage proteins in barley. Heredity 50, 179–189.Google Scholar
Shewry, P. R., Bunce, N. A. C., Kreis, M. & Forde, B. J. (1985 a). Polymorphism at the Hor 1 locus of barley (Hordeum vulgare L.). Biochemical Genetics 23, 389–402.Google Scholar
Shewry, P. R., Kreis, M., Parmar, S., Lew, E. J.-L. & Kasarda, D. D. (1985 b). Identification of γ-type hordeins in barley. FEBS Letters 190, 61–64.Google Scholar
Shewry, P. R. & Miflin, B. J. (1982). Genes for the storage proteins of barley. Qualitas Plantarum. Plant Foods for Human Nutrition 31, 251–267.Google Scholar
Shewry, P. R. & Parmar, S. (1987). The Hrd F (Hor 5) locus encodes γ-type hordeins. Barley Genetics Newsletter 17. In press.Google Scholar
Shewry, P. R., Williamson, M. S. & Kreis, M. (1987). Effects of mutant genes on the synthesis of storage components in developing barley endosperms. In Mutant Genes that Affect Plant Development (ed. Thomas, H.), pp. 95–118. Cambridge: Cambridge University Press.Google Scholar