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Map-based isolation of disease resistance genes from bread wheat: cloning in a supersize genome

Published online by Cambridge University Press:  23 June 2005

BEAT KELLER
Affiliation:
Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, 8008 Zurich, Switzerland
CATHERINE FEUILLET
Affiliation:
UMR INRA-UBP ASP Clermont-Ferrand, 234 av. du Brézet, 63039 Clermont-Ferrand Cedex 2, France
NABILA YAHIAOUI
Affiliation:
Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, 8008 Zurich, Switzerland
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Abstract

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The genome of bread wheat is hexaploid and contains 1·6×1010 bp of DNA, of which more than 80% is repetitive sequences. Its size and complexity represent a challenge for the isolation of agronomically important genes, for which we frequently know only their position on the genetic map. Recently, new genomic resources and databases from genome projects have simplified the molecular analysis of the wheat genome. The first genes to be isolated from wheat by map-based cloning include three resistance genes against the fungal diseases powdery mildew and leaf rust. In this review, we will describe the approaches and resources that have contributed to this progress, and discuss genomic strategies that will simplify positional cloning in wheat in the near future.

Type
Minireview
Copyright
© 2005 Cambridge University Press