Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-09T07:06:35.463Z Has data issue: false hasContentIssue false
  • English
  • Français

Maintenance of kappa particles in cells recently deprived of gene K (stock 51, syngen 4) of Paramecium aurelia*

Published online by Cambridge University Press:  14 April 2009

K. K. Yeung
K. K. Yeung
Affiliation:
Institute of Animal Genetics, Edinburgh, 9
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Heterozygous (K/k) killer paramecia containing kappa particles from stock 51, syngen 4 of Paramecium aurelia were passed through autogamy, and the kk offspring tested for killing ability and for presence of stainable kappa particles at various fission stages.

2. After eight fissions, a small proportion of the paramecia were non-killers, and a few lacked stainable particles. After the 12th fission nearly all cells lacked kappa.

3. Mild starvation of the kk kappa-bearing paramecia resulted in complete loss of kappa and conversion to non-killers, even when applied to animals only one fission after the loss of gene K at autogamy.

4. The results obtained here show some discrepancy with data obtained earlier, both with kappa, and with mu particles in stock 540, syngen 1. The reasons for this discrepancy are at present not clear.

Type
Research Article
Information
Genetics Research , Volume 6 , Issue 3 , November 1965 , pp. 411 - 418
Copyright
Copyright © Cambridge University Press 1965

References

REFERENCES

Austin, M. L. (1948). The killing action and rate of production of single particles of paramecin 51. Physiol. Zoöl. 21, 69–86.CrossRefGoogle ScholarPubMed
Chao, P. K. (1953). Kappa concentration per cell in relation to the life cycle, genotype, and mating type in Paramecium aurelia, variety 4. Proc. natn. Acad. Sci. U.S.A. 39, 103–12.Google Scholar
DeLamater, E. C. (1951). A staining and dehydrating procedure for the handling of microorganisms. Stain Technol. 26, 199.Google Scholar
Dryl, S. (1959). Antigenic transformation in Paramecium aurelia after homologous antiserum treatment during autogamy and conjugation. J. Protozool. 6 Suppl.25.Google Scholar
Gibson, I. & Beale, G. H. (1962). The mechanism whereby the genes M 1 and M 2 in Paramecium aurelia, stock 540, control growth of the mate-killer (mu) particles. Genet. Res. 3, 24–50.Google Scholar
Sonneborn, T. M. (1939). Paramecium aurelia: mating types and groups; lethal interactions; determination and inheritance. Am. Nat. 73, 390–413.CrossRefGoogle Scholar
Sonneborn, T. M. (1943). Gene and cytoplasm. I. The determination and inheritance of the killer character in variety 4 of Paramecium aurelia. II. The bearing of determination and inheritance of characters in P. aurelia on problems of cytoplasmic inheritance, pneumococcus transformations, mutations and development. Proc. natn. Acad. Sci. U.S.A. 29, 329–43.CrossRefGoogle Scholar
Sonneborn, T. M. (1947). Recent advances in the genetics of Paramecium and Euplotes. Adv. Genet. 1, 264.Google ScholarPubMed