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Macronuclear DNA in Stentor coeruleus: a first approach to its characterization

Published online by Cambridge University Press:  14 April 2009

B. Pelvat  and
G. De Haller
B. Pelvat
Affiliation:
Laboratoire de Biologie des Protistes, Département de Biologie Animale, Université de Genève, 1211 Genève 4 (Suisse)
G. De Haller
Affiliation:
Laboratoire de Biologie des Protistes, Département de Biologie Animale, Université de Genève, 1211 Genève 4 (Suisse)
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The macronuclei of Stentor coeruleus were isolated on a discontinuous sucrose gradient and their DNA was purified by conventional methods. The GC content was 32 mole%. The DNA banded as a single peak on analytical ultracentrifugation at 1·691 g/cm3. The molecular weight of the DNA was 5 × 106 to 4 × 107 daltons. Genome size determined by DNA–DNA reassociation kinetics was 6 × 1010 daltons. The macronuclear genome was mostly simple, about 85% being made of non-repetitive sequences.

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 2 , April 1976 , pp. 277 - 289
Copyright
Copyright © Cambridge University Press 1976

References

Allen, S. L. & Gibson, I. (1971). The purification of DNA from the genomes of Paramecium aurelia and Tetrahymena pyriformis. Journal of Protozoology 18, 518–525.CrossRefGoogle Scholar
Allen, S. L. & Gibson, I. (1972). Genome amplification and gene expression in the ciliate macronucleus. Biochemical Genetics 6, 293–313.CrossRefGoogle ScholarPubMed
Ammermann, D., Steinbrueck, G., von Berger, L. & Henning, W. (1974). The development of the macronucleus in the ciliates Protozoa Stylonychia mytilus. Chromosoma 45, 401–429.CrossRefGoogle Scholar
Barnett, A. & Zadylak, S. In Van Wagtendonk, W. J. (1974). Paramecium: A Current Survey. Elsevier Scientific Publishing Company.Google Scholar
Britten, R. J. (1971). Sequence complexity, kinetic complexity and genetic complexity. Carnegie Institution Yearbook 69, 503–506.Google Scholar
Britten, R. J. & Kohne, D. E. (1966). Nucleotide sequence repetition in DNA. Carnegie Institution Yearbook 65, 78–125.Google Scholar
Britten, R. J. & Kohne, D. E. (1968). Repeated sequences in DNA. Science 161, 529–540.CrossRefGoogle ScholarPubMed
Burgi, E. & Hershey, A. (1963). Sedimentation rate as a measure of molecular weight of DNA. Biophysical Journal 3, 309–321.CrossRefGoogle ScholarPubMed
Cairns, J. (1963). The chromosome of E. coli. Cold Spring Harbor Symposium on Quantitative Biology 28, 43.CrossRefGoogle Scholar
Chrispeels, M. J. (1973). Molecular Techniques and Approach in Developmental Biology. London: Wiley-Interscience.Google Scholar
Cummings, D. J. (1972). Isolation and partial characterization of macro- and micronuclei from P. aurelia. Journal of Cell Biology 53, 105–115.CrossRefGoogle Scholar
Davila, C., Charles, P. & Ledoux, L. (1965). The chromatography of nucleic acid preparation on DEAE cellulose paper. Journal of Chromatography 19, 382–395.CrossRefGoogle ScholarPubMed
Davis, R. W., Simon, M. & Davidson, N. (1971). Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods in Enzymology 21 B, 413–428.CrossRefGoogle Scholar
de Haller, G. & Rouiller, E. (1961). La structure fine de Chlorogonium elongaten Etude systématique au M. électronique. Journal of Protozoology 8, 452–462.CrossRefGoogle Scholar
de Terra, N. (1974). Cortical control of cell division. Science 184, 4163.CrossRefGoogle ScholarPubMed
Ehret, C. F. & Powers, E. L. (1955). Macronuclear and nucleolar development in Paramecium bursaria. Experimental Cell Research 9, 241–257.CrossRefGoogle ScholarPubMed
Engberg, J., Nilsson, J. R., Pearlman, R. E. & Leick, V. (1974). Induction of nuclear and mitochondrial DNA replication in Tetrahymena. Proceeding of the National Academy of Sciences, U.S.A. 71, 895–898.CrossRefGoogle Scholar
Flavell, R. A. & Jones, I. G. (1970). Kinetic complexity of Tetrahymena pyriformis nuclear desoxyribonucleic acid. Biochemical Journal 116, 155–157.CrossRefGoogle Scholar
Gall, J. G. (1974). Free ribosomal RNA in the macronucleus of Tetrahymena. Proceeding of the National Academy of Sciences, U.S.A. 71, 3078–3081.CrossRefGoogle ScholarPubMed
Gavurin, L. & Hirshfield, H. (1974). Synthesis and inhibition of macronuclear DNA in regenerating Blepharisma. Journal of Experimental Zoology 187, 387–404.CrossRefGoogle Scholar
Giese, A. C. (1973). Blepharisma. The Biology of a Light-sensitive Protozoan. Stanford University Press.Google Scholar
Hough, B. R. & Davidson, E. N. (1972). Studies on the repetitive sequence transcripts of Xenopus. Journal Molecular Biology 70, 491–509.CrossRefGoogle ScholarPubMed
Jurand, A., Beale, G. H. & Young, M. R. (1962). Studies on the macronucleus of Paramecium aurelia. Journal of Protozoology 9, 122–131.CrossRefGoogle ScholarPubMed
Kirby, K. S. (1968). Isolation of nucleic acids with phenolic solvents. Methods in Enzymology 12 b, 87–99.CrossRefGoogle Scholar
McCarthy, B. (1965). In Chandra & Appel (1973). Methoden der Molekularbiologie. Stuttgart: G. Fischer Verlag.Google Scholar
Mandel, M. & Marmur, J. (1968). Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods in Enzymology 12 B, 195–206.CrossRefGoogle Scholar
Mandel, M. & Muzyka, M. (1971). Desoxyribonucleic acids base composition of Stentor coeruleus. Journal of Protozoology 18, 139–141.CrossRefGoogle Scholar
Mandel, M., Schildkraut, C. L. & Marmur, J. (1968). Use of CsCl gradient analysis for determining the guanine plus cytosine content of DNA. Methods in Enzymology 12 B, 184–195.CrossRefGoogle Scholar
Marmur, J. & Doty, P. (1962). Determination of the base composition of DNA from its thermal denaturation temperature. Journal of Molecular Biology 5, 109–118.CrossRefGoogle ScholarPubMed
Mingot, L. & Davila, C. A. (1974). Chromatographic determination of the molecular weight of DNA. Journal of Chromatography 94, 75–83.CrossRefGoogle ScholarPubMed
Miyagishi, A. & Andoh, T. (1973). The DNA of Tetrahymena pyriformis GL strain: a mild method for preparation and its characterization. Biochimica et Biophysica Acta 299, 507–515.CrossRefGoogle Scholar
Miyake, A. & Beyer, J. (1973). Cell interaction by means of soluble factors (genome) in conjugation of Blepharisma intermedium. Experimental Cell Research 76, 1–15.CrossRefGoogle Scholar
Murti, K. G., Prescott, D. M. & Pene, J. J. (1972). DNA inciliate protozoan. III. Binding of RNA polymerase and denaturation at the ends of the low M.w. DNA in Stylonychia. Journal of Molecular Biology 68, 413–416.CrossRefGoogle Scholar
Paulin, J. J. (1973). Macronuclear reorganization during regeneration of oral membranelles in Stentor coeruleus. In Progress in Protozoology (abstract), 4th International Congress, UER, Clermont-Ferrand.Google Scholar
Peck, R., Pelvat, B., Bolivar, I. & De Haller, G. (1975). Light and electron microscopic observations on the heterotrich ciliate Climacostomum virens. Journal of Protozoology 22, 368–385.CrossRefGoogle Scholar
Pelvat, B., Hyvert, N. & De Haller, G. (1973). Synthèses d'acides nucléiques dans le macronoyau de Stentor coeruleus: Régénération et cycle de division. Protistologica 9, 255–267.Google Scholar
Prescott, D. M., Bostock, C. I., Murti, K. G., Lauth, M. R. & Gamow, E. (1971). DNA in ciliated protozoa. I. Electron microscopic and sedimentation analysis of macro- and micro-nuclear DNA of Stylonychia mytilus. Chromosoma 34, 355–366.CrossRefGoogle Scholar
Prescott, D. M., Murti, K. G. & Bostock, C. J. (1973). Genetic apparatus of Stylonychia. Nature 242, 576–600.CrossRefGoogle ScholarPubMed
Raikov, I. B. & Ammermann, D. (1974). The macronucleus of ciliates: Recent advances. In Actualités Protozoologiques, 4th International Congress, 1973 (ed. de Puytorae, P. and Grain, J.). UER Sciences, Clermont-Ferrand.Google Scholar
Rao, M. V. (1968). Nuclear behaviour of Spirostomum ambiguum during conjugation with special reference to macronuclear development. Journal of Protozoology 15, 748–752.CrossRefGoogle ScholarPubMed
Schildkraut, C. L., Mandel, M., Levisohn, S., Smith-Sonneborn, J. & Marmur, J. (1962). DNA base composition and taxonomy of some protozoan. Nature 196, 795–796.CrossRefGoogle Scholar
Soldo, A. & Godoy, G. A. (1972). The kinetic and complexity of Paramecium macronuclear DNA. Journal of Protozoology 19, 673–678.CrossRefGoogle Scholar
Sonneborn, T. M. (1947). Recent advances in the genetics of Paramecium and Euplotes. Advances in Genetics 1, 264–358.Google ScholarPubMed
Sonneborn, T. M. (1953). Electron micrographs of ultra-thin sections of the nucleic of P. aurelia. Microbiological and Genetical Bulletin 7, 24.Google Scholar
Sonneborn, T. M. (1975). Paramecium aurelia. In: Handbook of Genetics, vol. 2. Plenum Press.Google Scholar
Sueoka, N. (1961). Compositional correlation between DNA and protein. Cold Spring Harbor Symposium on Quantitative Biology 26, 35–44.CrossRefGoogle Scholar
Tartar, V. (1961). The Biology of Stentor. Oxford: Pergamon Press.CrossRefGoogle Scholar
Tobla, A. M., Brown, E. H., Parker, R. Y. & Schildkraut, C. L. (1972). DNA replication in synchronized cultured mammalian cells. Biophysica et Biochemica Acta 277, 256–268.Google Scholar
Wesley, R. D. (1975). Inverted repetitious sequences in the macronuclear DNA of hypotrichous ciliates. Proceedings of the National Academy of Sciences, U.S.A. 72, 678–682.CrossRefGoogle ScholarPubMed
Wetmur, J. G. & Davidson, N. (1968). Kinetics of renaturation of DNA. Journal of Molecular Biology 31, 349–370.CrossRefGoogle ScholarPubMed
Yao, M. C. & Gorovsky, M. A. (1974). Comparison of the sequences of macro- and micro-nuclear DNA of Tetrahymena pyriformis. Chromosoma 48, 1–18.CrossRefGoogle Scholar