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Longitudinal microtubules and their functions during asexual reproduction in Paramecium tetraurelia

Published online by Cambridge University Press:  14 April 2009

V. Sundararaman  and
Earl D. Hanson
V. Sundararaman
Affiliation:
Shanklin Laboratory, Wesleyan University, Middletown, Conn. 06457, U.S.A.
Earl D. Hanson
Affiliation:
Shanklin Laboratory, Wesleyan University, Middletown, Conn. 06457, U.S.A.
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Longitudinal microtubules appear in Paramecium tetraurelia when cells are ready to divide (ca. interfission age 0·9). They arise in the longitudinal cortical ridges between kineties and form, in each ridge, an incomplete circlet of 15–18 microtubules, disappearing 10–15 min after separation of the fission products (ca. interfission age 0·03–0·04). Vinblastine, colchicine, colcemid, mercaptoethanol, and cold treatments all result in loss of these microtubules, rounding up of the cells, and some suppression of cell division. The tubules are thought to play a role in cellular elongation, morphogenesis, and separation.

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 2 , April 1976 , pp. 205 - 211
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

Allen, R. D. (1967). Fine structure, reconstruction and possible functions of components of the cortex of Tetrahymena pyriformis. Journal of Protozoology 14, 553–565.CrossRefGoogle ScholarPubMed
Allen, R. D. (1971). Fine structure of membranous and microfibrillar systems in the cortex of Paramecium caudatum. Journal of Cell Biology 49, 1–20.CrossRefGoogle ScholarPubMed
Allen, R. D. (1974). Food vacuole membrane growth with microtubule-associated membrane transport in Paramecium. Journal of Cell Biology 63, 904–922.CrossRefGoogle ScholarPubMed
Bensch, K. G. & Malawista, S. E. (1969). Microtubular crystals in mammalian cells. Journal of Cell Biology 40, 95–107.CrossRefGoogle ScholarPubMed
Bryan, J. (1971). Vinblastine and microtubules. I. The induction and isolation of crystals from sea urchin oocytes. Experimental Cell Research 66, 129–136.CrossRefGoogle ScholarPubMed
Bryan, J. (1974). Microtubules. BioScience 24, 701–711.CrossRefGoogle Scholar
Burnside, B. (1971). Microtubules and microfilaments in newt neurulation. Developmental Biology 26, 416–441.CrossRefGoogle Scholar
Ehret, C. F., Abinger, J. & Savage, N. (1964). Developmental and ultrastructural studies of cell organelles. Argonne National Laboratory of Biology and Medicine Research Division Annual Report 6971, 62–70.Google Scholar
Ehret, C. F. & McArdle, E. W. (1974). The structure of Paramecium as viewed from its constituent levels of organization. In Paramecium: A Current Survey (ed. van Wagtendonk, W. J.), pp. 265–338. Amsterdam: Elsevier Scientific Publishing Company.Google Scholar
Hanson, E. D. (1962). Morphogenesis and regeneration of oral structures in Paramecium aurelia: An analysis of intracellular development. Journal of Experimental Zoology 150, 45–67.CrossRefGoogle ScholarPubMed
Hanson, E. D. (1974). Methods in the cellular and molecular biology of Paramecium. In Methods in Cell Biology (ed. Prescott, D. M.), pp. 319–365. New York: Academic Press.Google Scholar
Hufnagel, L. (1969). Cortical ultrastructure of Paramecium aurelia. Journal of Cell Biology 40, 779–801.CrossRefGoogle ScholarPubMed
Jurand, A. & Selman, G. G. (1969). The Anatomy of Paramecium aurelia. London: Macmillan; New York: St Martin Press.Google Scholar
Kaneda, M. & Hanson, E. D. (1974). Growth patterns and morphogenetic events in the cell cycle of Paramecium aurelia. In Paramecium: A Current Survey (ed. van Wagtendonk, W. J.), pp. 219–262. Amsterdam: Elsevier Scientific Publishing Company.Google Scholar
Kennedy, J. R. (1969). The role of microtubules in the cell cycle. In The Cell Cycle (ed. Padilla, G. M., Whitson, G. L. and Cameron, I. L.), pp. 227–248. New York: Academic Press.CrossRefGoogle Scholar
Kennedy, J. R. & Zimmerman, A. M. (1970). The effects of high hydrostatic pressure on the microtubules of Tetrahymena pyriformis. Journal of Cell Biology 47, 568–576.CrossRefGoogle ScholarPubMed
Marsland, D. A. & Landau, J. V. (1954). The mechanism of cytokinesis: temperature pressure studies on the cortical gel systems in various marine eggs. Journal of Experimental Zoology 125, 507–539.CrossRefGoogle Scholar
Schroeder, T. E. (1970). The contractile ring. I. Fine structure of dividing mammalian (HeLa) cells and the effects of cytochalasin B. Zeitschrift für Zellforschung und Mikroskopische Anatomie 109, 431–449.CrossRefGoogle Scholar
Selman, G. G. & Perry, M. M. (1970). Ultrastructural changes in the surface layers of the newt's egg in relation to the mechanism of its cleavage. Journal of Cell Science 6, 207–227.CrossRefGoogle Scholar
Sibley, J. & Hanson, E. D. (1974). Identity and function of a subcortical cytoskeleton in Paramecium. Archiv für Protistenkunde 116, 221–235.Google Scholar
Sonneborn, T. M. (1975). The Paramecium aurelia complex of fourteen sibling species. Transactions of the American Microscopical Society 94, 155–178.CrossRefGoogle Scholar
Sundararaman, V. & Hanson, E. D. (1974). The cortical microtubules and their functions during binary fission in P. aurelia. Journal of Protozoology 21, (suppl.) 6.Google Scholar
Szallosi, D. (1970). Cortical cytoplasmic filaments of cleaving eggs: a structural element corresponding to the contractile ring. Journal of Cell Biology 44, 192–209.CrossRefGoogle Scholar
Tilney, L. G. (1971). Origin and continuity of microtubules. In Origin and Continuity of Cell Organelles (ed. Reinert, J. and Ursprung, H.), pp. 222–260. New York: Springer-Verlag.CrossRefGoogle Scholar
Tilney, L. G. & Gibbins, J. R. (1969). Microtubules in the formation and development of the primary mesenchyme in Arbacia punctulata. II. The experimental analysis of their role in development and maintenance of cell shape. Journal of Cell Biology 41, 227–250.CrossRefGoogle ScholarPubMed
Tilney, L. G. & Porter, K. R. (1967). Studies on the microtubules in Heliozoa. II. The effect of low temperature on the formation and maintenance of the axopodia. Journal of Cell Biology 34, 327–343.CrossRefGoogle ScholarPubMed
Tucker, J. B. (1971). Microtubules and a contractile ring of microfilaments associated with a cleavage furrow. Journal of Cell Science 8, 557–571.CrossRefGoogle Scholar