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Linkage map of random amplified polymorphic DNAs (RAPDs) in the silkworm, Bombyx mori

Published online by Cambridge University Press:  14 April 2009

Amornrat Promboon ,
Toru Shimada ,
Haruhiko Fujiwara  and
Masahiko Kobayashi
Amornrat Promboon
Affiliation:
Department of Agrobiology, Faculty of Agriculture, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan
Toru Shimada
Affiliation:
Department of Agrobiology, Faculty of Agriculture, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan
Haruhiko Fujiwara
Affiliation:
Zoological Institute, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan
Masahiko Kobayashi
Affiliation:
Department of Agrobiology, Faculty of Agriculture, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan
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We have constructed a linkage map of random amplified polymorphic DNAs (RAPDs) in Bombyx mori. We screened 320 10-mer primers, and found 243 clear polymorphic bands between C108 and p50 strains. In the F2 generation, segregation ratios of 168 bands were nearly 3:1 in a chi square test, showing Mendelian inheritance. The MAPMAKER program sorted 168 bands into 29 linkage groups and 10 unlinked loci at minimum LOD score 3·0, and determined orders of loci in each group, which contained 2–11 markers. It also detected typing errors in our data. We calculated map distances between pairs of neighbouring loci using recombination values in males and the Kosambi mapping function. Our RAPD map consists of 169 loci including the p locus, and the sum of map distances is approximately 900 cM. Linkage groups 1 and 2 of our map correspond to chromosomes 1 and 2 on the conventional linkage map because of linkage to sex and p, respectively.

Type
Research Article
Information
Genetics Research , Volume 66 , Issue 1 , August 1995 , pp. 1 - 7
Copyright
Copyright © Cambridge University Press 1995

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