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The lactose system in Klebsiella aerogenes V9A 2. Galactoside permeases which accumulate lactose or melibiose

Published online by Cambridge University Press:  14 April 2009

E. C. R. Reeve  and
J. A. Braithwaite
E. C. R. Reeve
Affiliation:
Institute of Animal Genetics, West Mains Road, Edinburgh, EH9 9JN, Scotland
J. A. Braithwaite
Affiliation:
Institute of Animal Genetics, West Mains Road, Edinburgh, EH9 9JN, Scotland
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Klebsiella aerogenes V9A possesses at least three galactoside permeases, the lactose permease (LacP), the melibiose permease (MelP) and a third permease (GPIII), and also a galactose permease. It is shown that the first three of these permeases all accumulate both lactose and melibiose efficiently, while the galactose permease takes up neither sugar. Lactose only induces LacP and melibiose only induces MelP. d-fucose, isopropyl-β-d-thiogalactoside (IPTG) and methyl-β-d-thiogalactoside (TMG) all induce GPIII, but galactose does not. GPIII takes up both lactose and melibiose but cannot accumulate lactose in the presence of IPTG. Thus substantial differences are found between the galactoside permeases of Klebsiella and Escherichia coli.

Type
Research Article
Information
Genetics Research , Volume 21 , Issue 3 , June 1973 , pp. 273 - 285
Copyright
Copyright © Cambridge University Press 1973

References

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