Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-09T16:03:01.801Z Has data issue: false hasContentIssue false
  • English
  • Français

The isolation of mutants affecting ascus development in Neurospora crassa and their analysis by a zygote complementation test

Published online by Cambridge University Press:  14 April 2009

Adrian M. Srb  and
Mary Basl
Adrian M. Srb
Affiliation:
Section of Genetics, Development, and Physiology, Cornell University, Ithaca, New York, U.S.A.
Mary Basl
Affiliation:
Section of Genetics, Development, and Physiology, Cornell University, Ithaca, New York, U.S.A.
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A large number of mutants that affect the morphology of the ascus of Neurospora crassa has been isolated by means of a screening technique that utilizes the filtration principle to separate colonial type mutants from wild type. In relationships with each other and with wild-type alleles, the mutant genes exhibit zygote dominance and recessiveness. A zygote complementation test for placing the recessive mutants into functional groupings is described. Results obtained by use of the test place the mutants into seven functional groups. Dominance relations in the developing ascus of Neurospora are discussed.

Type
Research Article
Information
Genetics Research , Volume 13 , Issue 3 , June 1969 , pp. 303 - 311
Copyright
Copyright © Cambridge University Press 1969

References

REFERENCES

Beadle, G. W. & Tatum, E. L. (1945). Neurospora. II. Methods of producing and detecting mutations concerned with nutritional requirements. Am. J. Bot. 32, 678–686.CrossRefGoogle Scholar
De Terra, N. & Tatum, E. L. (1962). A relationship between cell wall structure and colonial growth in Neurospora crassa. Am. J. Bot. 50, 669–677.CrossRefGoogle Scholar
Dodge, B. O. (1927). Nuclear phenomena associated with homothallism and heterothallism in the ascomycete Neurospora. J. Agrie. Res. 35, 289–305.Google Scholar
Dodge, B. O., Singleton, J. R. & Rolnick, A. (1950). Studies on lethal E in Neurospora tetrasperma, including chromosome counts also in races of N. sitophila. Proc. Am. Phil. Soc. 94, 38–52.Google Scholar
Mitchell, M. B. (1966). A round-spore character in N. crassa. Neurospora Newsletter 10, 6.Google Scholar
Murray, J. C. & Srb, A. M. (1959). A recessive gene determining colonial growth of the mycelium and aberrant asci in Neurospora crossa. Proc. IX Int. Bot. Congr. 2, 276.Google Scholar
Murray, J. C. & Srb, A. M. (1962). The morphology and genetics of wild type and seven morphological mutant strains of Neurospora crossa. Can. J. Bot. 40, 337–349.Google Scholar
Perkins, D. D. (1959). New markers and multiple point linkage data in Neurospora. Genetics 44, 1185–1208.CrossRefGoogle ScholarPubMed
Srb, A. M. (1962). Genetic basis for an aberrant ascus in Neurospora crassa. Neurospora Newsletter 1, 6.Google Scholar
Woodward, V. W., DeZeeuw, J. R. & Srb, A. M. (1954). The separation and isolation of particular biochemical mutants of Neurospora by differential germination of conidia, followed by filtration and selective plating. Proc. natn. Acad. Sci. U.S.A. 40, 192–200.CrossRefGoogle ScholarPubMed