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Isolation of Bacillus subtilis transformation-deficient mutants and mapping of competence genes
Published online by Cambridge University Press: 14 April 2009
Summary
We have isolated and characterized 48 Bacillus subtilis competence-deficient mutants. The mutants, obtained by nitrososoguanidine mutagenesis or by insertional mutagenesis with transposon Tn917, had a reduced transformation frequency and a wild-type transduction frequency. The com mutations were mapped by PBS1 transduction and at least four new com genes have been identified. The mutants were also characterized for their capacity to bind and take up the transforming DNA.
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- Copyright © Cambridge University Press 1989
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