Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-19T06:26:58.407Z Has data issue: false hasContentIssue false
  • English
  • Français

Isolation and characterization of a cDNA clone corresponding to the mouse t-complex gene Tcp-1x

Published online by Cambridge University Press:  14 April 2009

Keith Dudley ,
Francis Shanahan ,
M. Burtenshaw ,
E. P. Evans ,
S. Ruddy  and
M. F. Lyon
Keith Dudley*
Affiliation:
Division of Biomolecular Sciences, King's College, Campden Hill Road, Kensington W8 7AH
Francis Shanahan
Affiliation:
Ludwig Institute for Cancer Research, St Mary's Hospital, Paddington, London W8
M. Burtenshaw
Affiliation:
William Dunn School of Pathology, South Parks Road, Oxford
E. P. Evans
Affiliation:
William Dunn School of Pathology, South Parks Road, Oxford
S. Ruddy
Affiliation:
MRC Radiobiology Unit, Chilton, Didcot, Oxon
M. F. Lyon
Affiliation:
MRC Radiobiology Unit, Chilton, Didcot, Oxon
*
* Corresponding author.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The mouse t complex on chromosome 17 is known to harbour many genes which have an important role in spermatogenesis. One of these, Tcp-1 has been cloned and shown to code for a protein probably essential for acrosome formation. During the isolation of a cDNA for Tcp-1 two other homologous sequences were recognized and described as Tcp-1x and Tcp-1y. In this paper we describe the isolation of a cDNA which has been shown by in situ hybridization to correspond to the Tcp-1x gene. Sequence analysis has confirmed that a 140 bp region of homology between Tcp-1 and Tcp-1x lies in the 3′ portion of both genes. Northern blotting has revealed that the Tcp-1x gene is expressed abundantly in liver where two transcripts are detectable and hybrid selection shows that the gene codes for a 37 kDa protein. A search of the DNA databases has failed to find any significant homology between Tcp-1x and any other sequences apart from Tcp-1.

Type
Research Article
Information
Genetics Research , Volume 57 , Issue 2 , April 1991 , pp. 147 - 152
Copyright
Copyright © Cambridge University Press 1991

References

Auffray, C. & Rougeon, F. (1980). Purification of mouse immunoglobulin heavy chain messenger RNAs from total myeloma tumor RNA. European Journal of Biochemistry 107, 303–314.CrossRefGoogle ScholarPubMed
Bennett, D. (1975). The T locus of the mouse. Cell 6, 441–454.CrossRefGoogle Scholar
Herrmann, B., Bucan, M., Mains, P. E., Frischauf, A.-M., Silver, L. & Lehrach, H. (1986). Genetic analysis of the proximal portion of the mouse t complex: evidence for a second inversion within t haplotypes. Cell 44, 469–476.CrossRefGoogle Scholar
Lyon, M. F., Zenthon, J., Evans, E. P., Burtenshaw, M. D., Dudley, K. & Willison, K. R. (1986). Location of the t complex on mouse chromosome 17 by in situ hybridisation. Immunogenetics 24, 125–127.CrossRefGoogle Scholar
Lyon, M. F., Zenthon, J., Evans, E. P., Burtenshaw, M. D. & Willison, K. R. (1988). Extent of the mouse t complex and its inversions shown by in situ hybridisation. Immunogenetics 27, 375–382.CrossRefGoogle Scholar
Rappold, G. A., Stubbs, L., Labeit, S., Crkvenjakov, R. B. & Lehrach, H. (1987). Identification of a testis-specific gene from the mouse t complex next to a CpG island. EMBO Journal 6, 1975–1980.CrossRefGoogle Scholar
Schimenti, J., Cebra-Thomas, J. A., Decker, C. L., Islam, S. D., Pilder, S. H. & Silver, L. M. (1988). A candidate gene for the mouse t complex responder (Tcr) locus responsible for haploid effects on sperm function. Cell 55, 71–78.CrossRefGoogle Scholar
Silver, L. M., Artzt, K. & Bennett, D. (1979). A major testicular cell protein specified by a mouse T/t complex gene. Cell 17, 275–284.CrossRefGoogle Scholar
Silver, L. M. (1985). Mouse t haplotypes. Annual Review of Genetics 19, 179–208.CrossRefGoogle ScholarPubMed
Willison, K. R., Dudley, K. & Potter, J. (1986). Molecular cloning and sequence analysis of a haploid expressed gene encoding t-complex polypeptide 1. Cell 44, 727–738.CrossRefGoogle ScholarPubMed
Willison, K., Lewis, V., Zuckerman, K. S., Cordell, J., Dean, C., Miller, K., Lyon, M. F. & Marsh, M. (1989). The t-complex polypeptide 1 (TCP-1) is associated with the cytoplasmic aspect of golgi membranes. Cell 57, 621–632.CrossRefGoogle ScholarPubMed