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Isolation and characterization of a cDNA clone corresponding to the mouse t-complex gene Tcp-1x

Published online by Cambridge University Press:  14 April 2009

Keith Dudley ,
Francis Shanahan ,
M. Burtenshaw ,
E. P. Evans ,
S. Ruddy  and
M. F. Lyon
Keith Dudley*
Affiliation:
Division of Biomolecular Sciences, King's College, Campden Hill Road, Kensington W8 7AH
Francis Shanahan
Affiliation:
Ludwig Institute for Cancer Research, St Mary's Hospital, Paddington, London W8
M. Burtenshaw
Affiliation:
William Dunn School of Pathology, South Parks Road, Oxford
E. P. Evans
Affiliation:
William Dunn School of Pathology, South Parks Road, Oxford
S. Ruddy
Affiliation:
MRC Radiobiology Unit, Chilton, Didcot, Oxon
M. F. Lyon
Affiliation:
MRC Radiobiology Unit, Chilton, Didcot, Oxon
*
* Corresponding author.

Summary

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The mouse t complex on chromosome 17 is known to harbour many genes which have an important role in spermatogenesis. One of these, Tcp-1 has been cloned and shown to code for a protein probably essential for acrosome formation. During the isolation of a cDNA for Tcp-1 two other homologous sequences were recognized and described as Tcp-1x and Tcp-1y. In this paper we describe the isolation of a cDNA which has been shown by in situ hybridization to correspond to the Tcp-1x gene. Sequence analysis has confirmed that a 140 bp region of homology between Tcp-1 and Tcp-1x lies in the 3′ portion of both genes. Northern blotting has revealed that the Tcp-1x gene is expressed abundantly in liver where two transcripts are detectable and hybrid selection shows that the gene codes for a 37 kDa protein. A search of the DNA databases has failed to find any significant homology between Tcp-1x and any other sequences apart from Tcp-1.

Type
Research Article
Information
Genetics Research , Volume 57 , Issue 2 , April 1991 , pp. 147 - 152
Copyright
Copyright © Cambridge University Press 1991

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