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Investigation of the mating system of Pseudomonas aeruginosa strain 1: I. Kinetic studies

Published online by Cambridge University Press:  14 April 2009

J. S. Loutit
Affiliation:
Department of Microbiology, University of Otago, Dunedin, New Zealand
L. E. Pearce
Affiliation:
Department of Microbiology, University of Otago, Dunedin, New Zealand
M. G. Marinus
Affiliation:
Department of Microbiology, University of Otago, Dunedin, New Zealand
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A method has been developed which will permit the mapping of the genetic material of Pseudomonas aeruginosa. This has been made possible by the use of potassium nitrate in the mating medium and other modifications which have increased the frequency of recombination. In certain circumstances, the ilvA12 marker may be transferred by more than 1% of the donor cells.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1968

References

REFERENCES

Davis, B. D. & Mingioli, E. S. (1950). Mutants of Escherichia coli requiring methionine or vitamin B 12. J. Bact. 60, 1728.CrossRefGoogle ScholarPubMed
De Haan, P. G. & Gross, J. D. (1962). Transfer delay and withdrawal during conjugation in Escherichia coli. Genet. Res. 3, 251272.CrossRefGoogle Scholar
Demerec, M., Adelberg, E. A., Clark, A. J. & Hartman, P. E. (1966). A proposal for a uniform nomenclature in bacterial genetics. Genetics 54, 6176.CrossRefGoogle ScholarPubMed
Holloway, B. W. (1955). Genetic recombination in Pseudomonas aeruginosa. J. gen. Microbiol. 13, 572581.Google ScholarPubMed
Holloway, B. W. & Fargie, B. (1960). Fertility factors and genetic linkages in Pseudomonas aeruginosa. J. Bact. 80, 362368.CrossRefGoogle ScholarPubMed
Loutit, J. S. & Marinus, M. G. (1967). Polarised chromosome transfer at relatively high frequency in Pseudomonas aeruginosa. Proc. Univ. Otago Med. Sch. 45, 1819.Google Scholar
Loutit, J. S. & Pearce, L. E. (1965 a). Mating in Pseudomonas aeruginosa. Nature, Lond. 205, 822.CrossRefGoogle ScholarPubMed
Loutit, J. S. & Pearce, L. E. (1965 b). Kinetics of mating of FP+ and FP strains of Pseudomonas aeruginosa. J. Bact. 90, 425430.CrossRefGoogle Scholar
Pittard, J., Loutit, J. S. & Adelberg, E. A. (1963). Gene transfer by F′ strains of Escherichia coli K 12. I. Delay in initiation of chromosome transfer. J. Bact. 85, 13941401.CrossRefGoogle ScholarPubMed