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Investigation of the mating system of Pseudodomonas aeruginosa strain I VI. Mercury resistance associated with the sex factor (FP)

Published online by Cambridge University Press:  14 April 2009

J. S. Loutit
J. S. Loutit
Affiliation:
Department of Microbiology, University of Otago, Dunedin, New Zealand
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Resistance to mercury has been shown to be associated with the presence of the sex factor (FP) in Pseudomonas aeniginosa. The resistance is expressed in all infected cells even though they do not necessarily function as donors.

For selection of freshly infected recipients when streptomycin or nalidixic acid were used for contra-selection of the donors, the level of mercury had to be reduced to between m/20000 and m/40000 when 500 units of streptomycin were present per ml and to m/40000 when 1·0 mg of nalidixic acid was included in each ml of medium. Normally donor strains would grow in the presence of Hg ions at a concentration of m/2000, which is 10–20 times greater than the concentration in the two selective media.

The kinetics of transfer of the sex factor were investigated and it was shown that transfer began almost as soon as the cells were mixed and was completed in 20 min when 5 min were allowed for pairing.

Type
Research Article
Information
Genetics Research , Volume 16 , Issue 2 , October 1970 , pp. 179 - 184
Copyright
Copyright © Cambridge University Press 1970

References

REFERENCES

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