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Interactions between the surface exclusion systems of some F-like plasmids

Published online by Cambridge University Press:  14 April 2009

Neil Willetts  and
John Maule
Neil Willetts
Affiliation:
MRC Molecular Genetics Unit, Department of Molecular Biology, University of Edinburgh, King's Buildings, Edinburgh EH9 3JR, Scotland
John Maule
Affiliation:
MRC Molecular Genetics Unit, Department of Molecular Biology, University of Edinburgh, King's Buildings, Edinburgh EH9 3JR, Scotland
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Four surface exclusion systems have been identified amongst a group of F-like plasmids in E. coli: SfxI(F), SfxII (ColV2 and R538-1fin−), SfxIII (ColVBtrp and R1-19) and SfxIV (R100-1 and R136fin−). None of these systems was expressed in stationary phase cells or, except for ColVBtrp, during fin+ transfer inhibition, showing that the surface exclusion gene(s) is usually co-controlled with the transfer genes.

Recipient cells carrying two plasmids specifying different surface exclusion systems did not always express both of these: the overall pattern suggested that the four systems and/or their sites of action are related. There was no surface exclusion between donor cells carrying two plasmids determining different surface exclusion systems and recipient cells carrying a plasmid determining either one of these. Our hypothesis to explain this and other results is that surface exclusion prevents interaction between the tip of the pilus on the donor cell and a receptor site on the recipient cell surface. Pili (probably mixed) with two types of tips would be present on cells carrying two different plasmids, the one unresponsive to the single surface exclusion system of the recipient cells allowing transfer of both plasmids.

Type
Research Article
Information
Genetics Research , Volume 24 , Issue 1 , August 1974 , pp. 81 - 89
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Achtman, M., Willetts, N. S. & Clark, A. J. (1971). Beginning a genetic analysis of conjugational transfer determined by the F factor in E. coli by isolation and characterization of transfer-deficient mutants. Journal of Bacteriology 106, 529–538.CrossRefGoogle Scholar
Achtman, M., Willetts, N. S. & Clark, A. J. (1972). Conjugational complementation analysis of transfer-deficient mutants of Flac in E. coli. Journal of Bacteriology 110, 831–842.CrossRefGoogle Scholar
Alfaro, G. & Willetts, N. S. (1972). The relationship between the transfer systems of some bacterial plasmids. Genetical Research 20, 279–289.CrossRefGoogle ScholarPubMed
Dennison, S. & Hedges, R. W. (1972). Host specificities of RNA phages. Journal of Hygiene, Cambridge, 70, 55–61.CrossRefGoogle ScholarPubMed
Finnegan, D. J. & Willetts, N. S. (1972). The nature of the transfer inhibitor of several F-like plasmids. Molecular and general Genetics 119, 57–66.CrossRefGoogle ScholarPubMed
Grindley, N. D. F., Grindley, J., Smith, H. R. & Anderson, E. S. (1973). Characterization of derepressed mutants of an F-like R factor. Molecular and general Genetics 120, 27–34.CrossRefGoogle ScholarPubMed
Lawn, A. M. & Meynell, E. (1970). Serotypes of sex pili. Journal of Hygiene 68, 683–694.Google ScholarPubMed
Lawn, A. M., Meynell, E. & Cooke, M. (1971). Mixed infections with bacterial sex factors: sex pili of pure and mixed phenotype. Annals Institut Pasteur 120, 3–8.Google ScholarPubMed
Le Blanc, D. J. & Falkow, S. (1973). Studies on superinfection immunity among transmissible plasmids in E. coli. Journal of Molecular Biology 74, 689–701.CrossRefGoogle Scholar
Lederberg, J., Cavalli, L. L. & Lederberg, E. M. (1952). Sex compatibility in E. coli. Genetics 37, 720–730.CrossRefGoogle Scholar
MacFarren, A. C. & Clowes, R. C. (1967). A comparative study of two F-like colicin factors, ColV2 and ColV3, in E. coli K12. Journal of Bacteriology 94, 365–377.CrossRefGoogle Scholar
Matsubara, K. (1968). Properties of sex factor and related episomes isolated from purified E. coli zygote cells. Journal of Molecular Biology 38, 89–108.CrossRefGoogle ScholarPubMed
Meynell, E. & Cooke, M. (1969). Repressor-minus and operator-constitutive derepressed mutants of F-like R factors: their effect on chromosomal transfer by HfrC. Genetical Research, 14, 309–313.CrossRefGoogle ScholarPubMed
Meynell, E. & Ewins, A. (1973). Effect on exclusion of alterations to the sex pilus. Journal of Bacteriology 113, 71–75.CrossRefGoogle Scholar
Novotny, C., Raizen, E., Knight, W. S. & Brinton, C. C. (1969). Functions of F-pili in mating-pair formation and male bacteriophage infection studied by blending spectra and reappearance kinetics. Journal of Bacteriology 98, 1307–1319.CrossRefGoogle Scholar
Ou, J. T. (1973 a). Inhibition of formation of E. coli mating pairs by f1 and MS2 bacteriophages as determined with a Coulter Counter. Journal of Bacteriology 114, 1108–1115.CrossRefGoogle ScholarPubMed
Ou, J. T. (1973 b). The F pilus conducts a signal for DNA synthesis between mating bacteria. Genetics 74, s206–s207.Google Scholar
Ou, J. T. (1974). Mating signal transmittance and surface exclusion in E. coli mating with minicells. Proceedings of the National Academy of Science, U.S.A., Submitted for publication.Google Scholar
Ou, J. T. & Anderson, T. F. (1972). Effect of Zn++ on bacterial conjugation: inhibition of mating pair formation. Journal of Bacteriology 111, 177–185.CrossRefGoogle ScholarPubMed
Sheehy, R. J., Orr, C. & Curtiss, R. (1972). Molecular studies on entry exclusion in E. coli minicells. Journal of Bacteriology 112, 861–869.CrossRefGoogle Scholar
Watanabe, T., Nishida, H., Ogata, C., Arai, T. & Sato, S. (1964). Episome-mediated transfer of drug resistance in Enterobacteriaceae VII. Two types of naturally occurring R factors. Journal of Bacteriology 88, 716–726.CrossRefGoogle ScholarPubMed
Willetts, N. S. (1971). Plasmid specificity of two proteins required for conjugation in E. coli K12. Nature New Biology 230, 183–185.CrossRefGoogle ScholarPubMed
Willetts, N. S. (1974). Mapping loci for surface exclusion and incompatibility on the F factor of E. coli K12. Journal of Bacteriology 118, 778–782.CrossRefGoogle Scholar
Willetts, N. S. & Finnegan, D. J. (1970). Characteristics of E. coli K12 strains carrying both an F prime and an R factor. Genetical Research 16, 113–122.CrossRefGoogle Scholar
Willetts, N. S. & Maule, J. (1973). Surface exclusion by ColV-K94. Genetical Research 21, 297–299.CrossRefGoogle ScholarPubMed