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Inheritance of protein amounts: comparison of two-dimensional electrophoresis patterns of leaf sheaths of two maize lines (Zea mays L.) and their hybrids

Published online by Cambridge University Press:  14 April 2009

A. Leonardi
Affiliation:
Laboratoire de Génétique des Systèmes Végétaux, La Ferme du Moulon, 91190 Gif-sur-Yvette, France
C. Damerval
Affiliation:
Laboratoire de Génétique des Systèmes Végétaux, La Ferme du Moulon, 91190 Gif-sur-Yvette, France
D. de Vienne
Affiliation:
Laboratoire de Génétique des Systèmes Végétaux, La Ferme du Moulon, 91190 Gif-sur-Yvette, France
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Denatured total proteins of two maize lines and their reciprocal F1 hybrids were analysed by two-dimensional electrophoresis. Quantitative (spot more or less intense) and qualitative (presence or absence of spots) differences exist between the lines, and correspond to 11% of the total reproducible spots. Non-additive effects on spot intensities were found in the hybrids, which display spots similar to the more intense parental spot for 11% of varying spots. This may correspond to dominance for regulatory systems controlling the protein amounts. Such interactions contrast with additivity classically described for enzymes or DNA.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1987

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