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The induction of ouabain-resistant mutants by N-methyl-N′-nitro-N-nitrosoguanidine in Chinese hamster cells

Published online by Cambridge University Press:  14 April 2009

Peter J. Davies  and
Jim Parry
Peter J. Davies
Affiliation:
Department of Genetics, University College of Swansea, Swansea SA2 8PP, U.K.
Jim Parry
Affiliation:
Department of Genetics, University College of Swansea, Swansea SA2 8PP, U.K.
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Summary

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Clones of V79 Chinese hamster cells resistant to the steroid compound ouabain have been induced by mutagen treatment with MNNG (= N-methyl-N′-nitro-N-nitrosoguanidine). The frequency of resistant colonies was increased from spontaneous value of 2 × 10−6 to a frequency of 1·2 × 10−3 with MNNG treatment.

The highest number of induced mutants appeared when an expression time pf 40 h was allowed between MNNG treatment and addition of ouabain.

Type
Research Article
Information
Genetics Research , Volume 24 , Issue 3 , December 1974 , pp. 311 - 314
Copyright
Copyright © Cambridge University Press 1974

References

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