Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-26T13:04:44.427Z Has data issue: false hasContentIssue false
  • English
  • Français

Induced recombination in the mitotic cell cycle of the yeast Saccharomyces cerevisiae

Published online by Cambridge University Press:  14 April 2009

Leland H. Johnston  and
Anthony L. Johnson
Leland H. Johnston
Affiliation:
Division of Microbiology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
Anthony L. Johnson
Affiliation:
Division of Microbiology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The occurrence of induced recombination in the mitotic cell cycle in yeast has been analysed using conditional cell-cycle mutants held at the restrictive temperature. The strains used were heteroallelic at gall and assaying for functional galactokinase shortly after irradiation (Johnston, 1982) allowed an unambiguous determination of the cell cycle stages in which recombination could occur. Recombination was observed in most strains, including those with the cdc36 mutation, defective in ‘start’; the cdc4, 7 and dbf4 mutations which arrest cells in G1; the dbf1, 2 and cdc6 mutations affecting S phase; cdc16 and cdc17 which block cells in G2 and also cdc14 and 15 which arrest cells in ‘late nuclear division’. Recombination can therefore occur within each of the major phases of the yeast cell cycle. This analysis has also revealed that the cdc8 mutation results in a defect in induced mitotic recombination.

Type
Research Article
Information
Genetics Research , Volume 43 , Issue 1 , February 1984 , pp. 1 - 10
Copyright
Copyright © Cambridge University Press 1984

References

REFERENCES

Arendes, J., Kim, K. C. & Sugino, A. (1983). Yeast 2 μm plasmid DNA replication in vitro: Purification of the CDC8 gene product by complementation assay. Proceedings of the National Academy of Sciences, U.S.A. 80, 673–677.CrossRefGoogle Scholar
Davies, P. J., Tippins, R. S. & Parry, J. M. (1978). Cell cycle variations in the induction of lethality and mitotic recombination after treatment with UV and nitrous acid in the yeast Saccharomyces cerevisiae. Mutation Research 51, 327–346.CrossRefGoogle ScholarPubMed
Esposito, M. S. (1978). Evidence that spontaneous mitotic recombination occurs at the two strand stage. Proceedings of the National Academy of Sciences, U.S.A. 75, 4436–4440.CrossRefGoogle ScholarPubMed
Esposito, R. E. (1968). Genetic recombination in synchronized cultures of Saccharomyces cerevisiae. Genetics 59, 181–210.CrossRefGoogle ScholarPubMed
Fabre, F. (1978). Induced intragenic recombination in yeast can occur during the G1 mitotic phase. Nature 272, 795–798.CrossRefGoogle ScholarPubMed
Fabre, F. & Roman, H. (1979). Evidence that a single DNA ligase is involved in replication and recombination in yeast. Proceedings of the National Academy of Sciences, U.S.A. 76, 4586–588.CrossRefGoogle ScholarPubMed
Glassberg, J., Meyer, R. R. & Kornberg, A. (1979). Mutant single-strand binding protein of E. coli: Genetic and physiological characterisation. Journal of Bacteriology 140, 14–19.CrossRefGoogle Scholar
Hartwell, L. H. (1974). Saccharomyces cerevisiae cell cycle. Bacteriological reviews 38, 164–198.CrossRefGoogle ScholarPubMed
Holliday, R. (1965). Induced mitotic crossing over in relation to genetic replication in synchronously dividing cells of Ustilago maydis. Genetical Research 6, 104–120.CrossRefGoogle ScholarPubMed
Johnston, L. H. (1977). The use of a novel plate assay in a search for yeast mutants defective in deoxyribonucleases. Molecular and General Genetics 152, 219–222.CrossRefGoogle Scholar
Johnston, L. H. (1982). Rapid detection of allelic recombination at the gall locus in yeast by assay of the recombinant gene product. Genetical Research 39, 85–97.CrossRefGoogle ScholarPubMed
Johnston, L. H. & Nasmyth, K. A. (1978). Saccharomyces cerevisiae cell cycle mutant cdc9 is defective in DNA ligase. Nature 274, 891–893.CrossRefGoogle ScholarPubMed
Johnston, L. H. & Thomas, A. (1982 a). The isolation of new DNA synthesis mutants in the yeast Saccharomyces cerevisiae. Molecular and General Genetics 186, 439–444.CrossRefGoogle ScholarPubMed
Johnston, L. H. & Thomas, A. (1982 b). A further two mutants defective in initiation of the S phase in the yeast Saccharomyces cerevisiae. Molecular and General Genetics 186, 445–448.CrossRefGoogle Scholar
Minet, M., Grossenbacher-Grunder, A. & Thuriaux, P. (1980). The origin of a centromere effect on mitotic recombination. A study in the fission yeast Schizosaccharomyces pombe. Current Genetics 2, 53–60.CrossRefGoogle ScholarPubMed
Reed, S. I. (1980). The selection of Saccharomyces cerevisiae mutants defective in the start event of cell division. Genetics 95, 561–577.CrossRefGoogle ScholarPubMed