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Induced expression of a Drosophila hsp70 promoter-fusion transgene is reduced after repeated heat shocks

Published online by Cambridge University Press:  14 April 2009

S. M. N. Hunt ,
M. R. Wilkins ,
H. W. Stokes ,
G. E. Daggard  and
R. Frankham
S. M. N. Hunt
Affiliation:
School of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia.
M. R. Wilkins
Affiliation:
School of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia.
H. W. Stokes
Affiliation:
School of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia.
G. E. Daggard
Affiliation:
School of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia.
R. Frankham*
Affiliation:
School of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia.
*
† Corresponding author.
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Summary

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Levels of transcripts produced by a heat shock protein 70 (hsp70)-antisense white transgene in Drosophila were measured after single and multiple heat shocks to determine whether the hsp70 promoter could produce sustained high levels of transgene transcripts. A single heat shock resulted in typical highly inducible levels of RNA, but the amount of antisense RNA was substantially reduced after multiple heat shocks. Endogenous hsp70 mRNA levels were also less abundant after multiple heat shocks as compared to a single heat shock. The hsp70 promoter is unsuitable for use in fusion gene constructs for long term expression studies where repeated heat shocks are required.

Type
Research Article
Information
Genetics Research , Volume 59 , Issue 3 , June 1992 , pp. 183 - 188
Copyright
Copyright © Cambridge University Press 1992

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