Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-07T06:28:21.247Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunogenetic analysis of proteins of Paramecium V. Detection of specific determinants in strains lacking a surface antigen*

Published online by Cambridge University Press:  14 April 2009

Irving Finger  and
Carol Heller
Irving Finger
Affiliation:
Biology Department, Haverford College, Haverford, Pennsylvania, U.S.A.
Carol Heller
Affiliation:
Biology Department, Haverford College, Haverford, Pennsylvania, U.S.A.
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Several stocks of Paramecium aurelia that had never expressed an E serotype were examined for E-reacting material. The regular presence of the cross-reacting antigen G in these stocks ruled out the use of hapten inhibition and other standard immunological techniques. Consequently, a genetic approach was attempted in which hybrids of the E-deficient strains and non-E stocks were bred, and antigen isolated and tested for the existence of molecules with a specificity differing from that of the E-containing parent.

Gel diffusion analysis suggested that E antigen from a hybrid indeed could differ from the ‘normal’ E. One of these hybrid clones which behaved as though its E antigen may have possessed a determinant contributed by the E-deficient strain was used as an immunizing antigen. The antibodies elicited possessed specificity unique for the hybrid. Thus the possession of E-like determinants by E-deficient stocks was confirmed.

Type
Research Article
Information
Genetics Research , Volume 5 , Issue 1 , February 1964 , pp. 127 - 136
Copyright
Copyright © Cambridge University Press 1964

References

REFERENCES

Austin, M. L., Widmayer, D. & Walker, L. M. (1956). Antigenic transformation as adaptive response of Paramecium aurelia to patulin; relation to cell division. Physiol. Zoöl. 19, 261–287.Google Scholar
Balbinder, E. & Peer, J. (1959). Gel diffusion studies on serotype and serotype transformation in Paramecium. J. gen. Microbiol. 21, 156–167.CrossRefGoogle ScholarPubMed
Beale, G. H. (1957). The antigen system of Paramecium aurelia. Int. rev. Cytol. 6, 1–23.Google Scholar
Bishop, J. O. & Beale, G. H. (1960). Genetical and biochemical studies of the immobilization antigens of Paramecium aurelia. Nature, Lond., 186, 734.CrossRefGoogle ScholarPubMed
Finger, I. (1957). The inheritance of the immobilization antigens of Paramecium aurelia, variety 2. J. Genet. 55, 361–374.Google Scholar
Finger, I. & Heller, C. (1960). Gel diffusion analysis of cross-reactions of a protein-hapten conjugate. J. Immunol. 85, 332–336.Google Scholar
Finger, I. & Heller, C. (1962). Immunogenetic analysis of proteins of Paramecium. I. Comparison of specificities controlled by alleles and by different loci. Genetics, 47, 223–239.CrossRefGoogle ScholarPubMed
Finger, I. & Heller, C. (1963). Immunogenetic analysis of proteins of Paramecium. IV. Evidence for the presence of hybrid antigens in heterozygotes. J. mol. Biol. 6, 190–202.Google Scholar
Finger, I., Heller, C. & Green, A. (1962). Immunogenetic analysis of proteins of Paramecium. II. Coexistence of two immobilization antigens within animals of a single serotype. Genetics, 47, 241–253.CrossRefGoogle ScholarPubMed
Hartmann, L. & Toilliez, M. (1957). Micro-méthode d'étude en gélose de la réaction antigène-anticorps (Variance du procédé d'Ouchterlony). Rev. franç., Etudes clin. biol. 2, 197–199.Google Scholar
Jones, I. G. & Beale, G. H. (1963). Chemical and immunological comparisons of allelic immobilization antigens in Paramecium aurelia. Nature, Lond., 197, 205–206.CrossRefGoogle Scholar
Ouchterlony, O. (1949). Antigen-antibody reactions in gels. Ark. Kemi B26, 1–19.Google ScholarPubMed
Preer, J. R. Jr (1956). A quantitative study of a technique of double diffusion in agar. J. Immunol. 77, 52–60.CrossRefGoogle ScholarPubMed
Preer, J. R. Jr (1959 a). Studies on the immobilization antigens of Paramecium. II. Isolation. J. Immunol. 83, 378–384.Google Scholar
Preer, J. R. Jr (1959 b). Studies on the immobilization antigens of Paramecium. IV. Properties of the different antigens. Genetics, 44, 803–814.Google Scholar
Sonneborn, T. M. (1948). The determination of hereditary antigenic differences in genically identical Paramecium cells. Proc.natl. Acad. Sci. Wash., 34, 413–418.CrossRefGoogle ScholarPubMed
Sonneborn, T. M. (1950). Methods in the general biology and genetics of Paramecium aurelia. J. exp. Zool. 113, 87–148.Google Scholar