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Identification of a second locus encoding β-amylase on chromosome 2 of barley

Published online by Cambridge University Press:  14 April 2009

M. Kreis ,
M. S. Williamson ,
P. R. Shewry ,
P. Sharp  and
M. Gale
M. Kreis*
Affiliation:
AFRC Institute of Arable Crop Research, Biochemistry Department, Rothamsied Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
M. S. Williamson
Affiliation:
AFRC Institute of Arable Crop Research, Biochemistry Department, Rothamsied Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
P. R. Shewry
Affiliation:
AFRC Institute of Arable Crop Research, Biochemistry Department, Rothamsied Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
P. Sharp
Affiliation:
AFRC Institute of Plant Science Research, Plant Breeding Institute. Mans Lane, Trumpington, Cambridge CB2 2LQ., U.K.
M. Gale
Affiliation:
AFRC Institute of Plant Science Research, Plant Breeding Institute. Mans Lane, Trumpington, Cambridge CB2 2LQ., U.K.
*
* Corresponding authors.
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Summary

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A barley endosperm cDNA clone was used to study the polymorphism and chromosomal location of β-amylase genes in barley. Analysis of DNA from seven cultivars digested with three restriction endonucleases showed two types of pattern, one present in Sultan and the other in the remaining six cultivars. A copy-number reconstruction indicated the presence of about three gene copies per haploid genome. Analysis of the six available whole chromosome addition lines and selected telocentric chromosome additions of barley into wheat showed the location of genes on the short arm of chromosome 2 (probably one copy) and the long arm of chromosome 4 (probably two copies).

Type
Research Article
Information
Genetics Research , Volume 51 , Issue 1 , February 1988 , pp. 13 - 16
Copyright
Copyright © Cambridge University Press 1988

References

Ainsworth, C. C., Miller, T. E. & Gale, M. D. (1987). α-amylase and β-amylase homoeoloci in species related to wheat. Genetical Research 49, 93–103.CrossRefGoogle Scholar
Ainsworth, C. C., Gale, M. D. & Baird, S. (1983). The genetics of β-amylase isoenzymes in wheat. 1. Allelic variation among hexaploid varieties and intra-chromosomal gene locations. Theoretical and Applied Genetics 66, 39–49.CrossRefGoogle Scholar
Allison, M. J. & Ellis, R. P. (1973). The inheritance of β-amylases in developing barley grain. Biochmeical Genetics 10, 165–173.CrossRefGoogle ScholarPubMed
Briggs, D. E. (1973). Hormones and carbohydrate metabolism in germinating cereal grains. In Biosynthesis and Its Control in Plants (ed. Milborrow, B. V.), pp. 219–277. London: Academic Press.Google Scholar
Hardie, D. G. (1975). Control of carbohydrase formation by gibberellic acid in barley endosperm. Phytochemisiry 14, 1719–1722.CrossRefGoogle Scholar
Islam, A. K. M. R., Shepherd, K. W. & Sparrow, D. H. B. (1975). Addition of individual barley chromosomes to wheat. Barley Genetics, III. Proceedings of the Third International Barley Generics Symposium, pp. 260–270. Garching, W. Germany.Google Scholar
Islam, A. K. M. R. (1983) Disomic additions of barley chromosomes to wheat. Proceedings of the Sixth International Wheat Genetics Symposium, pp. 233–238, Kyoto, Japan.Google Scholar
Kreis, M., Williamson, M., Buxton, B., Pywell, J., Hejgaard, J. & Svendsen, I. (1987). Primary structure and differential expression of β-amylase in normal and mutant barleys. European Journal of Biochemistry (In the Press.)CrossRefGoogle Scholar
Nielsen, G., Johansen, H., Jensen, J. & Hejgaard, J. (1983). Localization on barley chromosome 4 of genes coding for β-amylase (Bmy 1) and protein Z (Paz 1). Barley Genetics Newsletter 13, 55–57.Google Scholar
Powling, A., Islam, A. K. M. R. & Shepherd, K. W. (1981). Isozymes in wheat/barley hybrid derivative lines. Biochemical Genetics 19, 237–254.CrossRefGoogle ScholarPubMed
Sharp, P. J., Kreis, M., Shewry, P. R. & Gale, M. D. (1987). Location of β-amylase sequences in wheat and its relatives. Theoretical and Applied Genetics (In the Press.)Google Scholar
Visuri, K. & Nummi, M. (1972). Purification and charactenzation of crystalline β-amylase from barley, European Journal of Biochemistry 28, 555–565.CrossRefGoogle Scholar