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Heterogeneity of human haptoglobin α chains detected by two-dimensional gel electrophoresis

Published online by Cambridge University Press:  14 April 2009

Huw A. John
Affiliation:
Department of Genetics, University of Edinburgh, Edinburgh, Scotland
Ian F. Purdom
Affiliation:
Department of Genetics, University of Edinburgh, Edinburgh, Scotland
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Summary

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The protein spots representing the haptoglobin α1F, α1S and α2 chains in two-dimensional gels of human plasma samples representative of the six common haptoglobin phenotypes were identified by comparing their position with those of purified haptoglobin and distinguished from other spots in the vicinity by comparison with plasma with undetectably low levels of haptoglobin. Silver staining indicated that the α1F chain was represented by one spot in subtypes 1F-1F, 1F-1S and 1F-2, the α1S chain by three spots in subtypes 1S-1S, 1F-1S and 1S-2 and the α2 chain by six spots in 1F-2, 1S-2 and 2–2

Type
Research Article
Copyright
Copyright © Cambridge University Press 1987

References

Anderson, L. & Anderson, N. G. (1977). High resolution two-dimensional electrophoresis of human plasma proteins. Proceedings of the National Academy of Sciences, USA 74, 54215425.CrossRefGoogle ScholarPubMed
Anderson, L. & Anderson, N. G. (1979). Microheterogeneity of serum transferrin, haptoglobin and α2, HS glycoprotein examined by high resolution two-dimensional electrophoresis. Biochemical and Biophysical Research Communications 83, 258265.Google Scholar
Anderson, L. & Anderson, N. G. (1984). Some perspectives on two-dimensional protein mapping. Clinical Chemistry 30, 18981905.CrossRefGoogle ScholarPubMed
Bowman, B. H. & Kurosky, A. (1982). Haptoglobin: the evolutionary product of duplication, unequal crossing over, and point mutation. In Advances in Human Genetics, vol. 12 (ed. Harris, H. and Hirschhorn, K.), pp. 189261. New York: Plenum Publication Corporation.CrossRefGoogle Scholar
Brune, J. L., Yang, F., Barnett, D. R. & Bowman, B. H. (1984). Evolution of haptoglobin: comparison of complementary DNA encoding Hpα1S and Hpα2FS. Nucleic Acid Research 12, 45314538.Google Scholar
Daufeldt, J. A. & Harrison, H. H. (1984). Quality control and technical outcome of ISO-DALT two dimensional electrophoresis in a clinical laboratory setting. Clinical Chemistry 30, 19721980.CrossRefGoogle Scholar
John, H. A. & Purdom, I. F. (1984). Myelin proteins and collagen in the spinal roots and sciatic nerves of muscular dystrophic mice. Journal of the Neurological Sciences 65, 6980.Google Scholar
Laemlli, U. K. (1970). Cleavage of structural proteins during the assembly of bacteriophage T4. Nature 227, 680685.CrossRefGoogle Scholar
Margolis, J. & Kenrick, K. G. (1968). Polyacrylamide gel electrophoresis in a continuous molecular sieve gradient. Analytical Biochemistry 25, 347362.Google Scholar
McGill, J. R., Yang, F., Baldwin, W. D., Brune, J. L., Barnett, B. H. & Moore, C. M. (1984). Localization of the haptoglobin α and β genes (HPA and HPB) to chromosome 16q22 by in situ hybridization. Cytogenetics and Cell Genetics 38, 155157.CrossRefGoogle ScholarPubMed
Nance, W. E. & Smithies, O. (1963). New haptoglobin alleles: a prediction confirmed. Nature 198, 869870.CrossRefGoogle ScholarPubMed
O'Farrell, P. H. (1975). High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250, 40074021.Google Scholar
O'Farrell, P. Z., Goodman, H. M. & O'Farrell, P. H. (1977). High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12, 11331142.CrossRefGoogle ScholarPubMed
Putnam, F. W. (1975). Haptoglobin. In The Plasma Proteins, 2nd ed, vol. 2 (ed. Putnam, F. W.), pp. 150New York: Academic Press.Google Scholar
Sammons, D. W., Adams, L. D. & Nishizawa, E. E. (1981). A silver-based color development system for staining of polypeptides in polyacrylamide gels. Electrophoresis 2, 135141.CrossRefGoogle Scholar
Smithies, O., Connell, G. E. & Dixon, G. H. (1962 a). Inheritance of haptoglobin subtypes. American Journal of Human Genetics 14, 1421.Google ScholarPubMed
Smithies, O., Connell, G. E. & Dixon, G. H. (1962 b). Chromosomal rearrangements and the evolution of haptoglobin genes. Nature 196, 232236.Google Scholar
Teige, B., Olaisen, B. & Pedersen, L. (1985). Subtyping of haptoglobin – presentation of a new method. Human Genetics 70, 163167.Google Scholar
Van der Straten, A., Herzog, A., Cabezon, T. & Bollen, T. (1984). Characterization of human haptoglobin cDNAs coding for α2FS, β and α1S β variants. FEBS Letters 168, 103107.Google Scholar
Yang, H. J. & Przybylska, M. (1973). The microheterogeneity of human haptoglobin and its complex with haemoglobin. Canadian Journal of Biochemistry 51, 597605.Google Scholar