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A heat-shock-activated cDNA encoding GAGA factor rescues some lethal mutations in the Drosophila melanogaster Trithorax-like gene

Published online by Cambridge University Press:  29 August 2001

H. GRANOK ,
B. A. LEIBOVITCH  and
S. C. R. ELGIN
H. GRANOK
Affiliation:
Department of Biology, Campus Box 1229, Washington University, St Louis, MO 63130, USA Current address: Crossroads School, 500 DeBaliviere Ave, St. Louis, MO 63112, USA.
B. A. LEIBOVITCH
Affiliation:
Department of Biology, Campus Box 1229, Washington University, St Louis, MO 63130, USA
S. C. R. ELGIN
Affiliation:
Department of Biology, Campus Box 1229, Washington University, St Louis, MO 63130, USA
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Abstract

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GAGA factor is an important chromosomal protein involved in establishing specific nucleosome arrays and in regulating gene transcription in Drosophila melanogaster. We developed a transgenic system for controlled heat-shock-dependent overexpression of the GAGA factor 519 amino acid isoform (GAGA-519) in vivo. Efficient production of stable protein from these transgenes provided genetic rescue of a hypomorphic Trithorax-like (Trl) lethal allele to adulthood. Nevertheless, supplemental GAGA-519 did not suppress position effect variegation (PEV), a phenomenon commonly used to measure dosage effects of chromosomal proteins, nor did it rescue other lethal alleles of Trl. The results suggest requirements for the additional isoforms of GAGA factor, or for more precise regulation of synthesis, to carry out the diverse functions of this protein.

Type
Research Article
Information
Genetics Research , Volume 78 , Issue 1 , August 2001 , pp. 13 - 21
Copyright
2001 Cambridge University Press