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Haematopoietic role for Patch (Ph) revealed by new W mutant (Wct) in mice

Published online by Cambridge University Press:  14 April 2009

J. F. Loutit  and
B. M. Cattanach
J. F. Loutit
Affiliation:
M.R.C. Radiobiology Unit, Harwell, Didcot, Oxon, OX11 0RD
B. M. Cattanach
Affiliation:
M.R.C. Radiobiology Unit, Harwell, Didcot, Oxon, OX11 0RD
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A new mutant (Wct) has been identified at the W locus of the mouse. The homozygote is poorly viable. Whereas the heterozygote (Wct / +) is only mildly anaemic like Wυ / +, the double heterozygote Wct + / + Ph is considerably more anaemic than Wυ + / + Ph and it and Wsh + / + Ph have significantly raised leucocyte counts. Wct + / + Ph is also unduly radiosensitive to whole body X-irradiation, 50% dying from haematopoietic failure at a dose of 4·59 ± 0·14 Gy, whereas the median for Wct / + was 6·49 ± 0·28 Gy. Serial blood counts of mice after low- or sub-lethal doses of X-rays revealed significantly more profound depression of counts of both red cells and leucocytes in Wct +, and more notably in Wct + / + Ph, than in + / + or Wsh / + (haematologically normal) iso-dosed mice. We conclude that control of haematopoiesis by chromosome 5 is not confined to the W locus but is shared by the linked gene Ph (and perhaps Rw) and that expression of the change is not limited to the erythron but involves the pluripotent haematopoietic stem cell.

Type
Research Article
Information
Genetics Research , Volume 42 , Issue 1 , August 1983 , pp. 29 - 39
Copyright
Copyright © Cambridge University Press 1983

References

REFERENCES

Brown, J. A. H., Corp, M. J. & Westgarth, D. R. (1960). Effect of dose-rate and fractionation of X-ray dose on acute lethality in mice. International Journal of Radiation Biology 2, 371–381.Google Scholar
Cattanach, B. M. (1978). Another mutation at the W locus. Mouse News Letter 59, 18.Google Scholar
Cattanach, B. M. (1982). A further mutation at the W locus. Mouse News Letter 66, 64.Google Scholar
Cattanach, B. M., Iddon, C. A., Charlton, H. M., Chiappa, S. A. & Fink, G. (1977). Gonadotrophin-releasing hormone deficiency in a mutant mouse with hypogonadism. Nature 249, 338–340.CrossRefGoogle Scholar
Finney, D. J. (1952). Probit analysis, 2nd ed.Cambridge University Press.Google Scholar
Geissler, E. N., McFarland, E. C. & Russell, E. S. (1981). Analysis of pleiotropism at the dominant white spotting (W) locus of the mouse: a description of ten new alleles. Genetics 97, 337–361.Google Scholar
Grüneberg, H. & Truslove, G. M. (1960). Two closely linked genes in the mouse. Genetical Research 1, 69–90.CrossRefGoogle Scholar
Harrison, D. E. (1972). Life sparing ability (in lethally irradiated mice) of W/W υ mouse marrow with no macroscopic colonies. Radiation Research 52, 553–563.Google Scholar
Little, C. C. & Cloudman, A. M. (1937). The occurrence of a dominant spotting mutation in the house mouse. Proceedings of the National Academy of Sciences, USA 23, 535–537.CrossRefGoogle ScholarPubMed
Loutit, J. F., Corp, M. J. & Adams, P. J. V. (1982). Radiosensitivity of mice with mutations at loci W, Ph. International Journal of Radiation Biology 42, 93–97.Google Scholar
Loutit, J. F., Peters, J. & Marshall, M. J. (1981). Colony forming units and haematopoietic stem cells in osteoclastopoiesis. Metabolic Bone Disease and Related Research 3, 131–133.CrossRefGoogle ScholarPubMed
Lyon, M. F. & Glenister, P. H. (1982). A new allele sash (W sh) at the W locus and a spontaneous recessive lethal in mice. Genetical Research 39, 315–322.CrossRefGoogle Scholar
McCulloch, E. A., Siminovitch, L. & Till, J. E. (1964). Spleen-colony formation in anemic mice of genotype W/W υ. Science 144, 844–846.CrossRefGoogle Scholar
Russell, E. S. (1979). Hereditary anemias of the mouse: a review for geneticists. Advances in Genetics 20, 357–464.CrossRefGoogle Scholar
Russell, E. S., Bernstein, S. E., McFarland, E. C. & Modeen, W. R. (1963). The cellular basis of differential radiosensitivity of normal and genetically anemic mice. Radiation Research 20, 677–694.Google Scholar
Russell, E. S. & Bernstein, S. E. (1966). Blood and blood formation. In Biology of the Laboratory Mouse, 2nd ed. (ed. Green, E. L.), pp. 357–372. New York: McGraw-Hill.Google Scholar
Searle, A. G. (1965). Rump-white. Mouse News Letter 32, 39.Google Scholar