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Growth hormone and insulin-like growth factor-I measurements in high growth (hg) mice

Published online by Cambridge University Press:  14 April 2009

J. F. Medrano ,
D. Pomp ,
L. Sharrow ,
G. E. Bradford ,
T. R. Downs  and
L. A. Frohman
J. F. Medrano*
Affiliation:
Department of Animal Science, University of California, Davis, CA 95616-8521
D. Pomp
Affiliation:
Department of Animal Science, University of California, Davis, CA 95616-8521
L. Sharrow
Affiliation:
Department of Animal Science, University of California, Davis, CA 95616-8521
G. E. Bradford
Affiliation:
Department of Animal Science, University of California, Davis, CA 95616-8521
T. R. Downs
Affiliation:
Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio 45267-0547
L. A. Frohman
Affiliation:
Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio 45267-0547
*
* Corresponding author.
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Effects of a recessive gene causing high growth (hg) were studied on two major components of the growth axis in mice. Plasma and pituitary levels of growth hormone and plasma levels of insulin-like growth factor I (IGF-I) were measured in three lines homozygous for hg, each compared with a control line of alike genetic background but wild type for the hg locus (Hg). Line Gh (hghg) and line GH (HgHg) are from a line which had undergone long-term selection for high postweaning weight gain; line Ch (hghg) and line CH (HgHg) were extracted from the second backcross of Gh to C57BL/6J; line L54 (hghg) was from the sixth backcross to C57BL/6J (B6) (HgHg). Pituitary GH levels and plasma IGF-I levels were measured in both sexes at 3, 4·5, 6 and 9 wk of age. Plasma growth hormone was measured in 8- to 12-wk-old males at hourly intervals from 08.00 to 17.00. Body weight in lines homozygous for hg at 6 and 9 wk of age was 10–30% greater than in control lines. The ontogeny of this increased growth depended on genetic background. Pituitary growth hormone content was 52% lower in the two hghg lines measured (lines Ch and Gh) than in control lines at 4·5, 6 and 9 wk. Plasma growth hormone levels were also much lower in hg mice, with values only 20–30% of those in their respective controls, hg lines showed consistently low plasma growth hormone levels throughout the 9 hr sampling period, while control lines expressed the characteristic pulsatile hormone secretion. In contrast, plasma IGF-I levels were greater in line Ch (hghg) than in line CH (HgHg) at 3, 4·5 and 9 wk, and were also greater in line Gh (hghg) vs. line GH (HgHg) at 6 wk of age. The results suggest that the growth enhancing effect of the hg gene occurs through an IGF-I-mediated process. In addition, the genetic background itself is also a factor in the phenotypic expression of the gene.

Type
Research Article
Information
Genetics Research , Volume 58 , Issue 1 , August 1991 , pp. 67 - 74
Copyright
Copyright © Cambridge University Press 1991

References

Barria, N. & Bradford, G. E. (1981). Long-term selection for rapid gain in mice. I. Genetic analysis at the limit of response. Journal of Animal Science 52, 729–738.CrossRefGoogle ScholarPubMed
Berelowitz, M., Szabo, M., Frohman, L. A., Firestone, S., Chu, L. & Hintz, R. L. (1981). Somatomedin-C mediates growth hormone negative feedback by effects on both the hypothalamus and the pituitary. Science 212, 1279–1281.CrossRefGoogle ScholarPubMed
Blair, H. T., McCutcheon, S. N., Mackenzie, D. D. S., Gluckman, P. D. & Ormsby, J. E. (1987). Variation in plasma concentration of insulin-like growth factor-1 and its covariation with liveweight in mice. Australian Journal of Biological Sciences 40, 287–293.CrossRefGoogle ScholarPubMed
Blair, H. T., McCutcheon, S. N., Mackenzie, D. D. S., Gluckman, P. D., Ormsby, J. E. & Breier, B. H. (1989). Responses to divergent selection for plasma concentrations of insulin-like growth factor-1 in mice. Genetical Research 53, 187–191.CrossRefGoogle ScholarPubMed
Blair, H. T., McCutcheon, S. N. & Mackenzie, D. D. S. (1990). Components of the somatotropic axis as predictors of genetic merit for growth. Ed. Hill, W. G., Thompson, R, & Woolliams, J. A., Proceedings 4th World Congress on Genetics Applied to Livestock Production XIV, 246–255.Google Scholar
Bradford, G. E. (1971). Growth and reproduction in mice selected for rapid body weight gain. Genetics 69, 499–512.CrossRefGoogle ScholarPubMed
Bradford, G. E. & Famula, T. R. (1984). Evidence for a major gene for rapid postweaning growth in mice. Genetical Research 44, 293–298.CrossRefGoogle Scholar
Burke, W. H. & Marks, H. L. (1982). Nonselected and selected broiler lines of chickens from hatch to eight weeks of age. Growth 46, 283–295.Google ScholarPubMed
Calvert, C. C., Famula, T. R., Bernier, J. F. & Bradford, G. E. (1985). Serial composition during growth in mice with a major gene for rapid postweaning gain. Growth 49, 246–257.Google Scholar
Calvert, C. C., Famula, T. R., Bernier, J. F., Khalaf, N. & Bradford, G. E. (1986). Efficiency of growth in mice with a major gene for rapid postweaning gain. Journal of Animal Science 62, 77–85.CrossRefGoogle Scholar
Charlton, H. M. (1984). Mouse mutants as models in endocrine research. Journal of Experimental Physiology 69, 655–676.CrossRefGoogle ScholarPubMed
D'Ercole, A. J. & Underwood, L. E. (1980). Ontogeny of somatomedin during development in the mouse. Developmental Biology 79, 33–45.CrossRefGoogle ScholarPubMed
Dilts, R. B. (1988). Ovulation rate and pre- and post- implantation survival in mice with a major gene for postweaning growth. M.Sc. Thesis, University of California, Davis.Google Scholar
Downs, T. R., Chomczynski, P. & Frohman, L. A. (1990). Effects of thyroid deficiency and replacement on rat hypothalamic growth hormone (GH)-releasing hormone gene expression in vivo are mediated by GH. Molecular Endocrinology 4, 402–408.CrossRefGoogle ScholarPubMed
Famula, T. R., Calvert, C. C., Luna, E. & Bradford, G. E. (1988). Organ skeletal growth in mice with a major gene for rapid postweaning growth. Growth Development and Aging 52, 145–150.Google Scholar
Frohman, L. A. & Bernardis, L. L. (1968). Growth hormone and insulin levels in weanling rats with ventromedial hypothalamic lesions. Endocrinology 82, 1125–1132.CrossRefGoogle ScholarPubMed
Goddard, C., Wilkie, R. S. & Dunn, I. C. (1988). The relationship between insulin-like growth factor-1, growth hormone, thyroid hormones and insulin in chickens selected for growth. Domestic Animal Endocrinology 5, 165–176.CrossRefGoogle ScholarPubMed
Huybrechts, L. M., King, D. B., Lauterio, T. J., Marsh, J. & Scanes, C. G. (1985). Plasma concentrations of somatomedin-C in hypophysectomized, dwarf and intact growing domestic fowl as determined by heterologous radio-immunoassay. Journal of Endocrinology 104, 233–239.CrossRefGoogle Scholar
Katakami, H., Arimura, A. & Frohman, L. A. (1986). Growth hormone releasing hormone stimulates hypothalamic somatostatin release: an inhibitory feedback effect on growth hormone secretion. Endocrinology 118, 1872–1877.CrossRefGoogle Scholar
Lauterio, T. J., Decuypere, E. & Scanes, C. G. (1986). Growth, protein synthesis and plasma concentrations of growth hormone, thyroxine and triiodothyronine in dwarf, control and growth-selected strains of broiler-type domestic fowl. Comparative Biochemical Physiology 83A, 627–632.CrossRefGoogle Scholar
Mathews, L. S., Hammer, R. E., Behringer, R. R., D'Ercole, A. J., Bell, G. I., Brinster, R. L. & Palmiter, R. D. (1988). Growth enhancement of transgenic mice expressing human insulin-like growt6h factor I. Endocrinology 123, 2827–2833.CrossRefGoogle ScholarPubMed
McKnight, B. J. & Goddard, C. (1989). The effect of food restriction on circulating insulin-like growth factor-I in mice divergently selected for high or low protein or fat body mass ratios. Comparative Biochemical Physiology 92A, 565–569.CrossRefGoogle Scholar
Norton, S. A., Zavy, M. T., Maxwell, C. V., Buchanan, D. S. & Breazile, J. E. (1989). Insulin, growth hormone, glucose, and fatty acids in gilts selected for rapid vs. slow growth rate. American Journal of Physiology 257, E554–560.Google ScholarPubMed
Shibasaki, T., Yamauchi, N., Hotta, M., Masuda, A., Imaki, T., Demura, H., Ling, N. & Shizume, K. (1986). In vitro release of growth hormone-releasing factor from rat hypothalamus: effect of insulin-like growth factor-1. Regulatory Peptides 15, 47–53.CrossRefGoogle ScholarPubMed
Sinha, Y. N., Salocks, C. B., Wickes, M. A. & Vanderlaan, W. P. (1977). Serum and pituitary concentrations of prolactin and growth hormone in mice during a twenty-four hour period. Endocrinology 100, 786–791.CrossRefGoogle ScholarPubMed
Tannenbaum, G. S. & Martin, J. B. (1976). Evidence for an endogenous ultradian rhythm governing growth hormone secretion in the rat. Endocrinology 98, 562–570.CrossRefGoogle ScholarPubMed
Tannenbaum, G. S., Guyda, H. J. & Posner, B. I. (1983). Insulin-like growth factors: A role in growth hormone negative feedback and body weight regulation via brain. Science 220, 77–79.CrossRefGoogle ScholarPubMed
Tannenbaum, G. S. & Ling, N. (1984). The interrelationship of growth hormone (GH)-releasing factor and somatostatin in generation of the ultradian rhythm of GH secretion. Endocrinology 115, 1952–1957.CrossRefGoogle ScholarPubMed
Teller, W. M. (1985). Genetic Disorders of the Anterior Pituitary Gland in Endocrine Genetics and Genetics of Growth (ed. Papadatos, C. J. and Bartsocas, C. S.), pp. 91–102. Alan Liss Inc.Google Scholar
Yamashita, S. & Melmed, S. (1987). Insulin-like growth factor I regulation of growth hormone gene transcription in primary rat pituitary cells. Journal of Clinical Investigations 79, 449–452.CrossRefGoogle ScholarPubMed