Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-07T11:24:55.953Z Has data issue: false hasContentIssue false
  • English
  • Français

The genetic implications of u.v. light exposure and liquid-holding post-treatment in the yeast Saccharomyces cerevisiae

Published online by Cambridge University Press:  14 April 2009

James M.  and
Elizabeth M. Parry
James M.
Affiliation:
Department of Genetics, University College, Swansea, U.K.
Elizabeth M. Parry
Affiliation:
Department of Genetics, University College, Swansea, U.K.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

After liquid-holding treatment in saline, cultures of yeast exposed to u.v. light showed an increased resistance to a second exposure to u.v. irradiation. This increase in resistance during a second series of u.v. exposures was correlated with an increase in the induction of intragenic recombinants. In contrast, no increase in the frequency of the intergenic recombinants or mutations to prototrophy could be detected during the second u.v. dose range. The results obtained could not be explained by the induction of meiosis during liquid holding or by changes in the timing of cell division after u.v. exposure.

A model of u.v. repair in yeast is postulated in which liquid-holding treatment results in changes in the proportions of lesions repaired by excision repair and recombination repair (respectively).

Type
Research Article
Information
Genetics Research , Volume 19 , Issue 1 , February 1972 , pp. 1 - 16
Copyright
Copyright © Cambridge University Press 1972

References

REFERENCES

Cox, B. S. & Bevan, E. A. (1962). Aneuploidy in yeast. New Phytologist 61, 342.CrossRefGoogle Scholar
Ganeson, A. K. & Smith, K. C. (1968). Dark recovery processes in Escherichia coli irradiated with ultraviolet light. I. Effect of rec-mutation on liquid holding recovery. Journal of Bacteriology 96, 365–373.CrossRefGoogle Scholar
Ganeson, A. K. & Smith, K. C. (1969). Dark recovery processes in Escherichia coli irradiated with ultraviolet light. II. Effect of UVR genes on liquid holding recovery. Journal of Bacteriology 97, 1129–1133.CrossRefGoogle Scholar
Harm, W. & Haefner, K. (1968). Decreased survival resulting from liquid-holding of UV-irradiated Escherichia coli and Schizosaccharomyces pombe. Photochemistry and Photobiology 8, 179–192.CrossRefGoogle Scholar
Howard-Flanders, P. (1968). DNA repair. Annual Review of Biochemistry 37, 175–200.CrossRefGoogle ScholarPubMed
Mortimer, R. K. & Hawthorne, D. C. (1963). Genetic mapping in Saccharomyces. Genetics 63, 165.Google Scholar
Parry, J. M. & Cox, B. S. (1968). The effects of dark holding and photoreactivation on ultraviolet light-induced mitotic recombination and survival in yeast. Genetical Research 12, 187.CrossRefGoogle ScholarPubMed
Parry, J. M. & Parry, E. M. (1969). The effects of UV-light post-treatments on the survival characteristics of 21 UV-sensitive mutants of Saccharomyces cerevisiae. Mutation Research 21, 545–556.CrossRefGoogle Scholar
Patrick, M. H. & Haynes, R. H. (1968). Repair-induced changes in yeast radiosensitivity. Journal of Bacteriology 95, 1350–1354.CrossRefGoogle ScholarPubMed
Patrick, M. H., Haynes, R. H. & Uretz, R. M. (1964). Dark recovery phenomena in yeast. I. Comparative effects with various inactivating agents. Radiation Research 21, 144–163.CrossRefGoogle ScholarPubMed
Pomper, S., Daniels, K. N. & McKee, D. M. (1954). Genetic analysis of polyploid yeast. Genetics 39, 343.CrossRefGoogle ScholarPubMed
Witkin, E. (1969). UV induced mutation and DNA repair. Annual Review of Genetics 3, 525–552.CrossRefGoogle Scholar