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Genetic fine-structure of the GA-1 locus in the higher plant Arabidopsis thaliana (L.) Heynh

Published online by Cambridge University Press:  14 April 2009

M. Koornneef ,
J. Van Eden ,
C. J. Hanhart  and
A. M. M. De Jongh
M. Koornneef
Affiliation:
Department of Genetics, Agricultural University, Generaal Foulkesweg 53 6703 BM Wageningen, The Netherlands
J. Van Eden
Affiliation:
Department of Genetics, Agricultural University, Generaal Foulkesweg 53 6703 BM Wageningen, The Netherlands
C. J. Hanhart
Affiliation:
Department of Genetics, Agricultural University, Generaal Foulkesweg 53 6703 BM Wageningen, The Netherlands
A. M. M. De Jongh
Affiliation:
Department of Genetics, Agricultural University, Generaal Foulkesweg 53 6703 BM Wageningen, The Netherlands
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Non-germinating gibberellin (GA) responsive mutants are a powerful tool to study genetic fine structure in higher plants. Nine alleles (EMS-and fast neutron-induced) of the ga-1 locus of Arabidopsis thaliana were tested in a complete half-diallel. No wild type ‘recombinants’ were found in the selfed progeny of 9 homoallelic combinations (in total 3 × 105 plants); in the progenies from the 36 selfed hetero allelics the wild type frequency ranged from zero to 6·6 × 10−4. These frequencies allowed the construction of an internally consistent map for five different sites representing eight alleles. The ninth allele covered three sites and thus behaved like an intragenic deletion. The estimate of the total genetic length of the ga-1 locus was 0·07 cM. The order of the sites was also clearly reflected by the association with proximal outside markers. On the assumption that wild type gametes predominantly arise from reciprocal events, it was shown that a cross-over within the ga-1 locus leads to positive interference in the adjacent region.

The results are discussed with respect to the mutagen used, the frequencies found in other plant and Drosophila genes, and the possible occurrence of gene conversion.

Type
Research Article
Information
Genetics Research , Volume 41 , Issue 1 , February 1983 , pp. 57 - 68
Copyright
Copyright © Cambridge University Press 1983

References

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