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Genetic experiments on sisomicin production by Micromonospora rosea

Published online by Cambridge University Press:  14 April 2009

István Gadó
Affiliation:
Institute for Drug Research, Budapest, Hungary
Antónia Bokány
Affiliation:
Institute for Drug Research, Budapest, Hungary
György Szvoboda
Affiliation:
Institute for Drug Research, Budapest, Hungary
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Summary

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Chromosomal localization of a gene involved in sisomicin production of Micromonospora rosea n.sp. was suggested by the results of matings between antibiotic producer and non-producer auxotrophic mutants. The frequency of producers among prototrophic recombinants varied with the chromosomal markers used for selection. The sis allele of the parent which was able to overgrow its partner in mixed culture predominated amongst the recombinants.

Type
Short Paper
Copyright
Copyright © Cambridge University Press 1985

References

REFERENCES

Beretta, M., Betti, M. & Polsinelli, M. (1977). Genetic recombination in Micromonospora. Journal of Bacteriology 107, 415–119.CrossRefGoogle Scholar
Bibb, M. J., Freeman, R. F. & Hopwood, D. A. (1977). Physical and genetical characterization of a second sex factor, SCP2, for Streptomyces coelicolor A3/2/. Molecular and General Genetics 154, 156166.CrossRefGoogle Scholar
Birnboim, H. C. & Doly, J. (1979). A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acid Research 7, 15131523.CrossRefGoogle ScholarPubMed
Chater, K. F. & Hopwood, D. A. (1983). Streptomyces genetics. In The Biology of the Actinomycetes (ed. Goodfellow, M. et al. ), pp. 229286. London: Academic Press.Google Scholar
Chater, K. F., Hopwood, D. A., Kieser, T. & Thompson, C. J. (1982). Gene cloning in Streptomyces. In Current Topics in Microbiology and Immunology, vol. 96 (ed. Henle, W. et al. ), pp. 6995. Berlin, Heidelberg: Springer-Verlag.Google Scholar
Dobritsa, A. P., Dobritsa, S. V. & Tanyshin, V. J. (1978). Isolation and characterization of a plasmid from Bacillus brevis var. G.-B. cells. Molecular and General Genetics 164, 195204.CrossRefGoogle ScholarPubMed
Hopwood, D. A. (1978). Extrachromosomally determined antibiotic production. Annual Review of Microbiology 32, 373392.CrossRefGoogle ScholarPubMed
Hopwood, D. A. (1979 b). Genetics of antibiotic production by actinomycetes. Journal of Natural Products 42, 596602.CrossRefGoogle ScholarPubMed
Hopwood, D. A. (1983). Actinomycete genetics and antibiotic production. In Biochemistry and Genetic Regulation of Commercially Important Antibiotics (ed. Vining, L. C.), pp. 123. Reading, Massachussetts: Addison-Wesley Publishing Comp.Google Scholar
Hopwood, D. A. & Wright, H. M. (1978). Bacterial protoplast fusion: recombination in fused protoplasts of Streptomyces coelicolor. Molecular and General Genetics 162, 307317.CrossRefGoogle ScholarPubMed
Kado, C. I. & Liu, S.-T. (1981). Rapid procedure for detection and isolation of large and small plasmids. Journal of Bacteriology 145, 13651373.CrossRefGoogle ScholarPubMed
Okanishi, M. (1979). Plasmid and antibiotic synthesis in Streptomyces. In Genetics of Industrial Microorganisms (ed. Sebek, O. K. and Laskin, A. I.), pp. 134140. Washington D.C.: American Society for Microbiology.Google Scholar
Parag, Y. & Goedeke, M. E. (1984). A plasmid of the sisomicin producer Micromonospora inyoensis. Journal of Antibiotics (Japan) 37, 10821084.CrossRefGoogle ScholarPubMed
Shaw, P. D. & Piwowarski, J. (1977). Effects of ethidium bromide and acriflavine on streptomycin production by Streptomyces bikiniensis. Journal of Antibiotics (Japan) 30, 404408.CrossRefGoogle ScholarPubMed
Szvoboda, Gy., Láng, T., Gadó, I., Ambrus, G., Kari, Cs., Fodor, K. & Alföldi, L. (1979). Fusion of Micromonospora protoplasts. In Advances in Protoplast Research: Proceedings of the 5th International Protoplast Symposium, Szeged, Hungary (ed. Ferenczy, L. and Farkas, G. L.), pp. 235240. Budapest: Akadémiai Kiadó.Google Scholar