Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-25T06:20:12.119Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic effects of acridines on Pseudomonas aeruginosa

Published online by Cambridge University Press:  14 April 2009

Vilma Stanisich  and
B. W. Holloway
Vilma Stanisich
Affiliation:
Faculty of Science, Monash University, Clayton, Victoria 3168, Australia*
B. W. Holloway
Affiliation:
School of Microbiology, University of Melbourne, Parkville, Victoria 3052, Australia
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Mutants of Pseudomonas aeruginosa male strains with altered mating properties have been obtained through treatment with nitrogen half mustards. The response varied with the type of male strain. A mutant of the infectious male PTO 13 was obtained which acts as a female strain and has apparently lost the FP sex factor. A mutant of the non-infectious strain 2 male mates not only with strain 1 females, but shows a thousand-fold increase in its ability to mate with strain 2 males, when it can act as either donor or recipient in conjugation. Derivatives of strain 1 males were obtained which had reduced recombinant forming ability.

While acriflavine is ineffective in producing such mutants, and has not been shown to cure male strains of their sex factor in P. aeruginosa, it is very effective in inhibiting infectious transfer of FP from FP+ to FP− strains. Furthermore, it markedly inhibits recombinant formation in 1 FP− × 1 FP+ crosses, and this latter effect is thought to be due to the inhibition of chromosome transfer by the male parent. In view of the almost complete lack of effect of AF on the normal growth of P. aeruginosa it is likely that the control of chromosome replication during vegetative cell division is different from the occurring during conjugation and that the FP factor is involved in this control during conjugation.

Type
Research Article
Information
Genetics Research , Volume 13 , Issue 1 , February 1969 , pp. 57 - 70
Copyright
Copyright © Cambridge University Press 1969

References

REFERENCES

Albert, A., Rubbo, S. D., Goldacre, B. J., Davey, M. E. & Stone, J. D. (1945). The influence of chemical constitution on antibacterial activity. II. A general survey of the acridine series. Br. J. exp. Path. 24, 160–192.Google Scholar
Ames, B. N. & Whitfield, H. J. (1966). Frameshift mutagenesis in Salmonella.Cold Spring Harb. Symp. quant. Biol. 31, 221–225.CrossRefGoogle ScholarPubMed
Arai, T. & Watanabe, T. (1967). Effects of acriflavine on the transfer of episomes and bacterial chromosome in Escherichia coli K-12. Genet. Res., Camb. 10, 241–249.CrossRefGoogle ScholarPubMed
Cuzin, F. & Jacob, F. (1966). Inhibition par les acridines du transfert genetique par les souches donatrices d'E. coli K 12. Ann. Inst. Pasteur 111, 427–436.Google Scholar
Hirota, Y. (1960). The effect of acridine dyes on mating type factors of E. coli. Proc. natn. Acad. Sci., U.S.A. 46, 57–64.Google Scholar
Holloway, B. W. (1955). Genetic recombination in Pseudomonas aeruginosa. J. gen. Microbiol. 13, 572–581.Google ScholarPubMed
Holloway, B. W. (1956). Self-fertility in Pseudomonas aeruginosa. J. gen. Microbiol. 15, 221–224.CrossRefGoogle ScholarPubMed
Holloway, B. W. & Fargie, B. (1960). Fertility factors and genetic linkage in Pseudomonas aeruginosa. J. Bacterial. 80, 362–368.CrossRefGoogle ScholarPubMed
Holloway, B. W. & Jennings, P. A. (1958). An infectious fertility factor for Pseudomonas aeruginosa. Nature, Lond. 181, 855–856.CrossRefGoogle ScholarPubMed
Rolfe, B. & Holloway, B. W. (1966). Alterations in host specificity of bacterial DNA after an increase in growth temperature of Pseudomonas aeruginosa. J. Bacteriol. 92, 43–48.CrossRefGoogle ScholarPubMed
Samaha, R. J., White, C. W. & Herremann, R. L. (1967). Effect of acridine orange upon an Hfr strain of Escherichia coli K 12 displaying unusual transfer kinetics. J. molec. Biol. 28, 513–530.CrossRefGoogle Scholar
Stanisich, V. & Holloway, B. W. (1968). Conjugation in Pseudomonas aeruginosa. Genetics (in Press).Google Scholar
Vogel, H. J. & Bonner, D. M. (1956). Acetylornithinase of E. coli: partial purification and some properties. J. biol. Chem. 218, 97–106.CrossRefGoogle ScholarPubMed