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The genetic control of hexokinase isozymes in wheat

Published online by Cambridge University Press:  14 April 2009

C. C. Ainsworth
Affiliation:
Plant Breeding Institute, Maris Lane, Trumpington, Cambridge CB2 2LQ, England
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In extracts of mature wheat grains, 13 hexokinase isozymes were distinguished by IEF. The genes controlling the production of five isozymes were located on chromosome arms 1BS, 1DS and 3BS by nullisomic analysis. The three loci, part of two homoeoallelic series (Hk-1 and Hk-2) are designated Hk-B1, Hk-D1 and Hk-B2 respectively. Analysis of chromosome 1D short-arm terminal deletions indicated the Hk-D1 locus to be located proximally to the glucose phosphate isomerase locus, Gpi-D1 on the shortarm. Three variant HK phenotypes were distinguished amongst 55 hexaploid wheats examined. Analysis of seven Chinese Spring/Agropyron elongatum chromosome addition lines showed that Ag. elongatum isozymes were expressed in the wheat background in additions IV and V.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1983

References

REFERENCES

Ainsworth, C. C., Gale, M. D. & Baird, S. (1983). The genetics of β-amylase isozymes in wheat. I. Allelic variation among hexaploid varieties and intrachromosomal gene locations. Theoretical and Applied Genetics 66, 3949.CrossRefGoogle Scholar
Ainsworth, C. C., Gale, M. D. & Baird, S. (1984). The genetic control of grain esterases in hexaploid wheat. 1. Allelic variation. Theoretical and Applied Genetics. (In the Press.)CrossRefGoogle ScholarPubMed
Chojecki, A. J. S. & Gale, M. D. (1982). Genetic control of glucose phosphate isomerase in wheat and related species. Heredity 49, 337347.CrossRefGoogle Scholar
Chojecki, A. J. S., Gale, M. D., Holt, L. M. & Payne, P. I. (1983). The intrachromosomal mapping of a glucose phosphate isomerase structural gene, using allelic variation among stocks of Chinese Spring wheat. Genetical Research 41, 221226.CrossRefGoogle Scholar
Dvorak, J. (1980). Homoeology between Agropyron elongatum chromosome arms with Triticum chromosomes. Canadian Journal of Genetics and Cytology 21, 243254.CrossRefGoogle Scholar
Dvorak, J. & Knott, D. R. (1974). Disomic and ditelosomic additions of diploid Agropyron elongatum chromosomes to Triticum aestivum. Canadian Journal of Genetics and Cytology 16, 399417.CrossRefGoogle Scholar
Feingold, D. S. & Avigad, G. (1980). Sugar nucleotide transformations in plants. In The Biochemistry of Plants, vol. 3, Carbohydrates, Structure and function (ed. Preiss, J.), pp. 101170. New York: Academic Press.Google Scholar
Gale, M. D., Law, C. N., Chojecki, A. J. S. & Kempton, R. A. (1983). Genetic control of &agr;-amylase production in wheat. Theoretical and Applied Genetice 64, 309316.CrossRefGoogle Scholar
Hart, G. E. (1970). Evidence for triplicate genes for alcohol dehydrogenase in hexaploid wheat.Proceedings of the National Academy of Sciences. U.S.A. 66, 11361141.CrossRefGoogle ScholarPubMed
Hart, G. E. (1982). Biochemical loci of hexaploid wheat (Triticum aestivum, 2n = 42, Genomes AABBDD). Genetics Maps 2, 373376.Google Scholar
Hart, G. E. & Tuleen, N. A. (1983). Chromosomal locations of eleven Elytrigia elangata (= Agropyron elongatum) isozyme structural genes. Genetical Research 41, 181202.CrossRefGoogle Scholar
Higgins, T. J. C. & Easterby, J. S. (1974). Wheatgerm hexokinase: physical and active-site properties. European Journal of Biochemistry 45, 147160.CrossRefGoogle ScholarPubMed
Lunderstadt, J. (1966). The effect of rust infection on hexokinase activity and carbohydrate dissimilation in primary leaves of wheat. Canadian Journal of Botany 44, 13451364.CrossRefGoogle Scholar
Meunier, J. C., Buc, J. & Ricard, J. (1971). Isolation, purification and characterization of wheat germ hexokinase. FEBS Letters 14, 2528.CrossRefGoogle Scholar
Nishikawa, K. & Nobuhara, M. (1971). Genetic studies of α-amylase isozymes in wheat. I. Location of genes and variation in tetra- and hexaploid wheat. Japanese Journal of Genetics 46, 345348.Google Scholar
Paulis, J. W. & Wall, J. A. (1979). Note on mill for pulverising single kernels of cereals for isoelectric focusing. Cereal Chemistry 56, 497498.Google Scholar
Payne, P. I., Holt, L. M., Worland, A. J. & Law, C. N. (1982). Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. Part III. Telocentric mapping of the subunit genes on the long arms of the homoeologous group 1 chromosomes. Theoretical and Applied Genetics 63, 129138.CrossRefGoogle Scholar
Payne, P. I., Holt, L. M., Jackson, E. A., Chojecki, A. J. S., Gale, M. D. & Bennett, M. D. (in preparation). Lack of expression of genes at the Gli-D1 locus, which code for endosperm storage proteins in one variety of wheat, two varietal biotypes and numerous landraces from Nepal.Google Scholar
Saltman, P. (1953). Hexokinase in higher plants. Journal of Biological Chemistry 200, 145154.CrossRefGoogle ScholarPubMed