Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-11-23T08:35:17.607Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic analysis of spontaneous half-sectored colonies of Saccharomyces cerevisiae

Published online by Cambridge University Press:  14 April 2009

J. R. Johnston
J. R. Johnston
Affiliation:
Department of Applied Microriology, University of Strathclyde, Glasgow C 1
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Genetic analysis of spontaneous half-sectored colonies of a diploid strain homozygous for ade2 and heterozygous for linked markers aro1A, hom2, ade8 and trp4 was undertaken. Some of these were due to an unexpectedly high rate of ade2 reversion and others to a low incidence of haploid sectors. About 50% resulted from mitotic recombination. Only a few were due to mitotic crossing over alone, the majority resulted from non-reciprocal ‘conversion’, including recombination extending over two loosely-linked loci, and some colonies featured both mitotic crossing over and mitotic conversion. The latter indicate that negative interference operates for mitotic recombination. The rate of mitotic recombination for ade8, calculated from the frequency of half-sectored colonies was 3·1 × 10−4 per cell division.

Type
Research Article
Information
Genetics Research , Volume 18 , Issue 2 , October 1971 , pp. 179 - 184
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Eposito, R. E. (1968). Genetic recombination in synchronized cultures of Saccharomyces cerevisiae. Genetics 59, 191–210.CrossRefGoogle Scholar
Fincham, J. R. S. & Holliday, R. (1970). An explanation of fine structure map expansion in terms of excision repair. Molecular and General Genetics 109, 309–322.CrossRefGoogle ScholarPubMed
Fogel, S. & Mortimer, R. K. (1969). Informational transfer in meiotic gene conversion. Proceedings of the National Academy of Sciences of the U.S.A. 62, 96–103.CrossRefGoogle ScholarPubMed
Fogel, S. & Mortimer, R. K. (1970). Fidelity of meiotic gene conversion in yeast. Molecular and General Genetics 109, 177–185.CrossRefGoogle Scholar
Hawthorne, D. C. & Mortimer, R. K. (1960). Chromosome mapping in Saccharomyces: centromere-linked genes. Genetics 45, 1085–1110.CrossRefGoogle ScholarPubMed
Hurst, D. D. & Fogel, S. (1964). Mitotic recombination and heteroallelic repair in Saccharomyces cerevisiae. Genetics 50, 435–458.CrossRefGoogle ScholarPubMed
James, A. P. & Lee-Whiting, B. (1955). Radiation-induced genetic segregations in vegetative cells of diploid yeast. Genetics 40, 826–831.CrossRefGoogle ScholarPubMed
Johnston, J. R. (1962). Studies in meiotic and mitotic recombination in Saccharomyces cerevisiae. Ph.D. thesis, University of California.Google Scholar
Johnston, J. R. & Mortimer, R. K. (1959). Use of snail digestive juice in isolation of yeast tetrads. Journal of Bacteriology 78, 292.CrossRefGoogle Scholar
Nakai, S. & Mortimer, R. K. (1969). Studies of the genetic mechanism of radiation-induced mitotic segregation in yeast. Molecular and General Genetics 103, 329–338.CrossRefGoogle ScholarPubMed
Pritchard, R. H. (1955). The linear arrangement of a series of alleles of Aspergittus nidulans. Heredity 9, 343–371.CrossRefGoogle Scholar
Pontecorvo, G. & Käfer, E. (1958). Genetic analysis based on mitotic recombination. Advances in Genetics 9, 71–104.CrossRefGoogle ScholarPubMed
Roman, H. (1956). A system selective for mutations affecting the synthesis of adenine in yeast. Compte rendu des travaux du Laroratoire de Carlsberg (Ser. Physiol.) 26, 299–314.Google Scholar
Roman, H. & Jacob, F. (1958). A comparison of spontaneous and ultraviolet-induced allelic recombination with reference to the recombination of outside markers. Cold Spring Harbor Symposia on Quantitative Biology 23, 155–160.CrossRefGoogle Scholar
Stahl, F. W. (1969). One way to think arout gene conversion. Genetics 61 (Suppl.), 1–13.Google Scholar
Thornton, R. J. & Johnston, J. R. (1971). Rates of spontaneous mitotic recombination in Saccharomyces cerevisiae. Genetical Research, Cambridge (in the Press).CrossRefGoogle Scholar
Wildenberg, J. (1970). The relation of mitotic recombination to DNA replication in yeast pedigrees. Genetics 66, 291–304.CrossRefGoogle ScholarPubMed
Wilkie, D. & Lewis, D. (1963). The effect of ultraviolet light on recombination in yeast. Genetics 48, 1701–1716.CrossRefGoogle ScholarPubMed
ZimmermannF. K., Schwaier, R. F. K., Schwaier, R. & Laer, V. U. (1966). Mitotic recombination induced in Saccharomyces cerevisiae with nitrous acid, diethylsulphate and carcinogenic, alkylating nitrosamides. Zeitschrift für Vererbungslehre 98, 230–246.Google Scholar