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A genetic analysis of resistance to nystatin in Saccharomyces cerevisiae

Published online by Cambridge University Press:  14 April 2009

K. A. Ahmed  and
R. A. Woods
K. A. Ahmed
Affiliation:
Department of Genetics, University of Sheffield
R. A. Woods
Affiliation:
Department of Genetics, University of Sheffield
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1. A number of stable nystatin-resistant mutants of the yeast Saccharomyces cerevisiae have been isolated from platings of a sensitive wild-type strain on low concentrations of the antibiotic.

2. These mutants were found to be resistant to 10, 15 or 60 units of drug/ml.

3. Analysis of meiotic segregants from crosses of these mutants to wild-type indicate that resistance is determined by two types of genes; resistance genes and modifiers.

4. Functional analysis of the mutants demonstrated the existence of three recessive resistance genes, nys-l, nys-2 and nys-3 and that nys-1 and nys-2 were linked.

5. Genetic analysis showed that nys-1 was affected by two modifiers, Mnys-1 and Mnys-2, but that only Mnys-2 affected nys-2 and nys-3.

6. The modifiers Mnys-1 and Mnys-2 are dominant.

7. An investigation of the effects of temperature and medium on resistance demonstrated marked interactions between genotype and environment for both the resistance genes and the modifiers.

8. Second-step mutants have been isolated by plating first-step mutants on higher concentrations of the drug. Some of these are resistant to 800 units/ml.

9. Some possible mechanisms of nystatin resistance are discussed.

Type
Research Article
Information
Genetics Research , Volume 9 , Issue 2 , April 1967 , pp. 179 - 193
Copyright
Copyright © Cambridge University Press 1967

References

REFERENCES

Bevan, E. A. & Costello, W. P. (1964). The preparation and use of an enzyme which breaks open yeast asci. Microb. Genet. Bull. 21, 5.Google Scholar
Bevan, E. A. & Woods, R. A. (1962). Complementation between adenine requiring mutants in yeast. Heredity, Lond. 17, 141.Google Scholar
Bryan, B. E. (1961). Genetic modifiers of streptomycin resistance in Pneumococcus. J. Bact. 82, 461–470.CrossRefGoogle ScholarPubMed
Cavalli, L. L. & Maccacaro, G. G. (1952). Polygenic inheritance of drug resistance in the bacterium Escherichia coli. Heredity, Lond. 6, 311–331.Google Scholar
Cox, B. S. & Bevan, E. A. (1962). Aneuploidy in yeast. New Phytol. 61, 342–355.Google Scholar
Dubnau, D. A. & Pollock, M. R. (1965). The genetics of Bacillus licheniformis penicillinase: a preliminary analysis from studies on mutation and inter-strain and intra-strain transformations. J. gen. Microbiol. 41, 7–21.Google Scholar
Hazen, G. L. & Brown, R. (1951). Fungicidin, an antibiotic produced by a soil Actinomycete. Proc. Soc. exp. Biol. Med. 76, 93–97.Google Scholar
Hebeka, E. K. & Solotorovsky, M. (1965). Development of resistance to polyene antibiotics in Candida albicans. J. Bact. 89, 1533–1539.CrossRefGoogle ScholarPubMed
Hawthorne, D. C. & Mortimer, R. K. (1960). Chromosome mapping in Saccharomyces: Centromere-linked genes. Genetics, 45, 1085–1110.Google Scholar
Kemp, R. F. O. & Bevan, E. A. (1959). New techniques for isolating spores of Hymenomycetes. Trans. Br. mycol. Soc. 42, 308–311.Google Scholar
Lampen, J. O. (1966). Interference by polyene antifungal antibiotics (especially nystatin and filipin) with specific membrane functions. Symp. Soc. gen. Microbiol. 16, 111–130.Google Scholar
Lampen, J. O., Arnow, P. M. & Safferman, R. S. (1960). Mechanism of protection by sterols against polyene antibiotics. J. Bact. 80, 200–206.CrossRefGoogle ScholarPubMed
Lampen, J. O., Arnow, P. M., Borowska, Z. & Laskin, A. I. (1962). Location and role of sterol at nystatin-binding sites. J. Bact. 84, 1152–1160.Google Scholar
Littman, M. L., Pisano, M. A. & Lancaser, R. M. (1958). Induced resistance of Candida species to nystatin and amphotericin B. Antibiotics A. 1957–58, 981–987.Google Scholar
Manning, L. K. & Robertson, B. M. (1959). A case of aspergillosis treated with nystatin. Br. Med. J. 1, 345–346.CrossRefGoogle ScholarPubMed
Marini, F., Arnow, P. M. & Lampen, J. O. (1961). The effect of monovalent cations on the inhibition of yeast metabolism by nystatin. J. gen. Microbiol. 24, 51–62.Google Scholar
Perkins, D. D. (1953). The detection of linkage in tetrad analysis. Genetics, 38, 187–197.Google Scholar
Richmond, M. H. (1965). Penicillinase plasmids in Staphylococcus aureus. Br. med. Bull. 21, 260–263.Google Scholar
Stanley, V. C. & English, M. P. (1965). Some effects of nystatin on the growth of four Aspergillus species. J. gen. Microbiol. 40, 107–118.Google Scholar
Stout, H. A. & Pagano, J. F. (1956). Resistance studies with nystatin. Antiobiotics A. 1955–56, 704–710.Google Scholar
Szybalski, W. (1952). Microbial selection. I. Gradient plate technique for the study of bacterial resistance. Science, N.Y. 116, 46–48.Google Scholar
van Zutphen, H., van Deenen, L. L. M. & Kinsky, S. C. (1966). The action of polyene antibiotics on bilayer lipid membranes. Biochem. biophys. Res. Commun. 22, 393–398.Google Scholar
Wilkie, D. & Lee, B. K. (1965). Genetic analysis of actidione resistance in Saccharomyces cerevisiae. Genet. Res. 6, 130–138.Google Scholar